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Titolo:
Vaxfectin enhances the humoral immune response to plasmid DNA-encoded antigens
Autore:
Hartikka, J; Bozoukova, V; Ferrari, M; Sukhu, L; Enas, J; Sawdey, M; Wloch, MK; Tonsky, K; Norman, J; Manthorpe, M; Wheeler, CJ;
Indirizzi:
Vical Inc, Dept Chem, San Diego, CA 92121 USA Vical Inc San Diego CA USA 92121 Inc, Dept Chem, San Diego, CA 92121 USA Vical Inc, Dept Cell Biol, San Diego, CA 92121 USA Vical Inc San Diego CAUSA 92121 Dept Cell Biol, San Diego, CA 92121 USA Vical Inc, Dept Pharmacol, San Diego, CA 92121 USA Vical Inc San Diego CAUSA 92121 Dept Pharmacol, San Diego, CA 92121 USA
Titolo Testata:
VACCINE
fascicolo: 15-16, volume: 19, anno: 2001,
pagine: 1911 - 1923
SICI:
0264-410X(20010228)19:15-16<1911:VETHIR>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNODEFICIENCY-VIRUS TYPE-1; DIRECT GENE-TRANSFER; SKELETAL-MUSCLE; IN-VIVO; INTRAMUSCULAR INJECTION; MONOCLONAL-ANTIBODIES; NONHUMAN-PRIMATES; MALARIA VACCINE; RABIES VIRUS; IMMUNIZATION;
Keywords:
genetic immunization; plasmid DNA; cationic lipid;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Wheeler, CJ Vical Inc, Dept Chem, 9373 Towne Ctr Dr, San Diego, CA 92121 USA Vical Inc 9373 Towne Ctr Dr San Diego CA USA 92121 A 92121 USA
Citazione:
J. Hartikka et al., "Vaxfectin enhances the humoral immune response to plasmid DNA-encoded antigens", VACCINE, 19(15-16), 2001, pp. 1911-1923

Abstract

This report characterizes Vaxfectin, a novel cationic and neutral lipid formulation which enhances antibody responses when complexed with an antigen-encoding plasmid DNA (pDNA). In mice, intramuscular injection of Vaxfectin formulated with pDNA encoding influenza nucleoprotein (NP) increased antibody titers up to 20-fold. to levels that could not be reached with pDNA alone. As little as 1 mug of pDNA formulated with Vaxfectin per muscle resultedin higher anti-NP titers than that obtained with 25 mug naked pDNA. The antibody titers in animals injected with Vaxfectin-pDNA remained higher than in the naked pDNA controls for at least 9 months. The enhancement in antibody titers was dependent on the Vaxfectin dose and was accomplished without diminishing the strong anti-NP cytolytic T cell response typical of pDNA-based vaccines. In rabbits, complexing pDNA with Vaxfectin enhanced antibody titers up to 50 fold with needle and syringe injections and also augmented humoral responses when combined with a needle-free injection device. Vaxfectin did not facilitate transfection and/or increase synthesis of beta -galactosidase reporter protein in muscle tissue. ELISPOT assays performed on bone marrow cells from vaccinated mice showed that Vaxfectin produced a three- to five-fold increase in the number of NP-specific plasma cells. Thus. Vaxfectin should be a useful adjuvant for enhancing pDNA-based vaccinations. (C) 7001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 19:10:45