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Titolo:
Enhancement of VP1-specific immune responses and protection against EMCV-Kchallenge by co-delivery of IL-12 DNA with VP1 DNA vaccine
Autore:
Suh, YS; Ha, SJ; Lee, CH; Sin, JI; Sung, YC;
Indirizzi:
Pohang Univ Sci & Technol, Dept Life Sci, Natl Res Lab, Pohang 790784, Kyungbuk, South Korea Pohang Univ Sci & Technol Pohang Kyungbuk South Korea 790784 South Korea
Titolo Testata:
VACCINE
fascicolo: 15-16, volume: 19, anno: 2001,
pagine: 1891 - 1898
SICI:
0264-410X(20010228)19:15-16<1891:EOVIRA>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
STIMULATORY FACTOR NKSF; MOUSE SKELETAL-MUSCLE; IN-VIVO TRANSFECTION; ENCEPHALOMYOCARDITIS VIRUS; DENDRITIC CELLS; SURFACE-ANTIGEN; MENGO-VIRUS; FACTOR GENE; D-VARIANT; CYTOKINE;
Keywords:
IL-12; EMCV VP1; protection; Th1/Th2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Sin, JI Pohang Univ Sci & Technol, Dept Life Sci, Natl Res Lab, San 31, Pohang 790784, Kyungbuk, South Korea Pohang Univ Sci & Technol San 31 Pohang Kyungbuk South Korea 790784
Citazione:
Y.S. Suh et al., "Enhancement of VP1-specific immune responses and protection against EMCV-Kchallenge by co-delivery of IL-12 DNA with VP1 DNA vaccine", VACCINE, 19(15-16), 2001, pp. 1891-1898

Abstract

It has been reported that co-delivery of IL-12 DNA with a DNA vaccine further enhances antigen (Ag)-specific protective immunity in pathogenic challenge models. However. the enhancing effects of antibody by IL-12 have been controversial. To clarify this issue, we constructed an IL-12 expression vector, co-immunized IL-12 DNA with an encephalomycocarditis virus (EMCV)-D VPI plasmid vaccine, and then evaluated immune modulatory effects and protection against lethal EMCV-K challenge. We observed that VP1-specific IgG production, as well as seroconversion rates, were significantly enhanced by IL-12 co-injection. indicating that IL-12 can enhance antibody responses in this model system. In particular, co-injection with VPI plus IL-12 DNA into the same leg enhanced systemic Ag-specific Ige production to a significantlygreater extent than either the separate leg injection of VPI and IL-12 DNAor VPI DNA vaccine alone. This suggests that local co-expression of IL-12 along with antigens is more important for enhanced antibody production. Furthermore, IgG2a isotype was significantly enhanced by IL-12 DNA co-injection. indicating a Th1 bias. In addition, co-delivery of IL-12 DNA was demonstrated to enhance VP1-specific Th cell proliferative responses. When animalswere challenged with a lethal dose of EMCV-K, IL-12 DNA-co-immunized animals exhibited enhanced survival, as compared to VP1 DNA vaccine alone. Thesestudies suggest that IL-12 plays an important role in increasing Ag-specific Th1 type antibody and cellular responses, resulting in enhanced protection against lethal EMCV-K challenge. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 11:10:02