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Titolo:
Diagnosis of cyanide intoxication by measurement of cytochrome c oxidase activity
Autore:
Ikegaya, H; Iwase, H; Hatanaka, K; Sakurada, K; Yoshida, K; Takatori, T;
Indirizzi:
Univ Tokyo, Grad Sch Med, Dept Forens Med, Bunkyo Ku, Tokyo 1130033, JapanUniv Tokyo Tokyo Japan 1130033 rens Med, Bunkyo Ku, Tokyo 1130033, Japan Natl Res Inst Police Sci, Kashiwa, Chiba 2770882, Japan Natl Res Inst Police Sci Kashiwa Chiba Japan 2770882 Chiba 2770882, Japan
Titolo Testata:
TOXICOLOGY LETTERS
fascicolo: 2, volume: 119, anno: 2001,
pagine: 117 - 123
SICI:
0378-4274(20010228)119:2<117:DOCIBM>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTAGONISM; MECHANISM; HEART; RAT;
Keywords:
cytochrome c oxidase; postmortem change; cyanide intoxication; postmortem diagnosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Ikegaya, H Univ Tokyo, Grad Sch Med, Dept Forens Med, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1130033, Japan Univ Tokyo 7-3-1 Hongo Tokyo Japan 1130033 okyo 1130033, Japan
Citazione:
H. Ikegaya et al., "Diagnosis of cyanide intoxication by measurement of cytochrome c oxidase activity", TOX LETT, 119(2), 2001, pp. 117-123

Abstract

Cytochrome c oxidase (CCO), a mitochondrial enzyme, is inactivated by cyanide or carbon monoxide (CO) intoxication. We measured CCO activity, in the major organs of the rat at various times after death caused by cyanide intoxication. Tissue samples were homogenized, and the CCO activity in the mitochondrial fraction was measured using ferrous cytochrome c as the substrate. The CCO activity inhibition was highest in the brain, although the cyanide concentration was lowest level. As a result of this and the clinical symptoms displayed, we consider the brain to be the primary organ of cyanide intoxication. As cyanide is highly toxic to humans, in small amounts and manypatients and victims have already had some medical care, it is difficult to detect cyanide in criminal investigations. The CCO activities in various organs remained significantly low for 2 days after the cyanide intoxication, suggesting that the diagnosis may be possible by measuring not only the cyanide concentration but also the CCO activity. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 20:37:52