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Titolo:
Expression of cyclin-dependent kinase inhibitors in taste buds of mouse and hamster
Autore:
Hirota, M; Ito, T; Okudela, K; Kawabe, R; Hayashi, H; Yazawa, T; Fujita, K; Kitamura, H;
Indirizzi:
Yokohama City Univ, Sch Med, Dept Pathol, Kanazawa Ku, Yokohama, Kanagawa 236004, Japan Yokohama City Univ Yokohama Kanagawa Japan 236004 Kanagawa 236004, Japan Yokohama City Univ, Sch Med, Dept Oral & Maxillofacial Surg, Kanazawa Ku, Yokohama, Kanagawa 236004, Japan Yokohama City Univ Yokohama Kanagawa Japan 236004 Kanagawa 236004, Japan
Titolo Testata:
TISSUE & CELL
fascicolo: 1, volume: 33, anno: 2001,
pagine: 25 - 32
SICI:
0040-8166(200102)33:1<25:EOCKII>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCLONAL-ANTIBODY KI-67; TERMINAL DIFFERENTIATION; POTENTIAL MEDIATOR; SKELETAL-MUSCLE; NUCLEAR ANTIGEN; CELL-TYPES; PROLIFERATION; PROTEIN; P27(KIP1); P53;
Keywords:
taste bud; immunohistochemistry; Western blotting; p21(Cip1); p21(Kip1);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Ito, T Yokohama City Univ, Sch Med, Dept Pathol, Kanazawa Ku, 3-9 Fuku Ura, Yokohama, Kanagawa 236004, Japan Yokohama City Univ 3-9 Fuku Ura YokohamaKanagawa Japan 236004 apan
Citazione:
M. Hirota et al., "Expression of cyclin-dependent kinase inhibitors in taste buds of mouse and hamster", TISSUE CELL, 33(1), 2001, pp. 25-32

Abstract

Taste buds are specialized epithelial cell clusters in the oral squamous cell epithelium. Although taste buds have been reported to renew rapidly, the mechanism of cell cycle control in these specialized structures remains unresolved, To clarify the cell cycle status and role of cyclin-dependent kinase inhibitors (CDKI) for cell cycle control in the taste buds, we analyzed cell proliferation activity using bromodeoxyuridine (BrdU) and Ki-67 immunostainings and the expression of the Cip/Kip family of CDKI (p21(Cip1), p27(Kip1), and p57(Kip2)) in the circumvallate papillae of mouse and hamster. BrdU-positive cells were detected in the basal layer of the oral epithelium. In the taste buds, Ki-67-positive cells were seen in the basal area, with only a very few positive cells in the taste buds. Both p21(Cip1) and p27(Kip1) positive cells were seen in the suprabasal layer of the non-gustatoryoral epithelium, In the taste buds, stronger p27(Kip1) staining was detected than in the non-gustatory epithelium. Western blotting analysis revealedthat p27(Kip1) was abundant in the mucosal tissues from circumvallate papillae, Thus, our study suggests that the taste bud cells except for basal cells are postmitotic cells and that the cell cycle arrest associated with taste bud cell differentiation could be regulated predominantly by p27(Kip1). (C) 2001 Harcourt Publishers Ltd.

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Documento generato il 30/09/20 alle ore 09:13:57