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Titolo:
Amiodarone stimulates interleukin-6 production in cultured human thyrocytes, exerting cytotoxic effects on thyroid follicles in suspension culture
Autore:
Nakajima, K; Yamazaki, K; Yamada, E; Kanaji, Y; Kosaka, S; Sato, K; Takano, K;
Indirizzi:
Tokyo Womens Med Univ, Inst Clin Endocrinol, Dept Med, Shinjuku Ku, Tokyo 1628666, Japan Tokyo Womens Med Univ Tokyo Japan 1628666 njuku Ku, Tokyo 1628666, Japan Kanaji Hosp, Kita Ku, Tokyo, Japan Kanaji Hosp Tokyo JapanKanaji Hosp, Kita Ku, Tokyo, Japan Tokyo Womens Med Univ, Gen Res Inst, Tokyo, Japan Tokyo Womens Med Univ Tokyo Japan Med Univ, Gen Res Inst, Tokyo, Japan
Titolo Testata:
THYROID
fascicolo: 2, volume: 11, anno: 2001,
pagine: 101 - 109
SICI:
1050-7256(200102)11:2<101:ASIPIC>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA EXPRESSION; INDUCED THYROTOXICOSIS; GENE-EXPRESSION; HORMONE; IODIDE; CELLS; IL-6; FIBROBLASTS; RECEPTOR; THERAPY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Sato, K Tokyo Womens Med Univ, Inst Clin Endocrinol, Dept Med, Shinjuku Ku, KawadaCho 8-1, Tokyo 1628666, Japan Tokyo Womens Med Univ Kawada Cho 8-1 Tokyo Japan 1628666 6, Japan
Citazione:
K. Nakajima et al., "Amiodarone stimulates interleukin-6 production in cultured human thyrocytes, exerting cytotoxic effects on thyroid follicles in suspension culture", THYROID, 11(2), 2001, pp. 101-109

Abstract

To investigate whether amiodarone increases interleukin-6 (IL-6) production in thyrocytes, human follicles obtained from subtotally thyroidectomized patients with Graves' disease were cultured in serum-free medium supplemented with various concentrations of bovine thyrotropin (bTSH) and amiodarone. The follicles gradually formed monolayer cells and secreted triiodothyronine (T-3), thyroglobulin (Tg), and IL-6 for at least 14 days. TSH dose-dependently increased T-3 and Tg but not IL-6 levels in the conditioned medium. Amiodarone exerted no significant effect on T-3, Tg, or IL-6 concentrationsat 0.1-1 muM. In contrast, at 10-20 muM, it decreased T-3 and Tg, but increased IL-6 levels, and these changes were accompanied by increased expression of IL-6 mRNA. Amiodarone-induced IL-6 production was inhibited by prednisolone at 10(-7) M. Electron microscopic examination revealed that the thyroid follicles in the suspension culture remained intact at 1 muM, but that cytotoxic effects (decreased microvilli and increased onion-like inclusion bodies) occurred at higher concentrations (10-25 muM). These in vitro findings indicate that amiodarone does not impair thyroid function at clinicallyattainable serum levels (1 muM), but exerts cytotoxic effect by inducing the production of a proinflammatory cytokine (IL-6) at higher concentrations. Because amiodarone-induced IL-6 production was inhibited by prednisolone,it is reasonable to administer glucocorticoids to patients with amiodarone-induced destructive thyrotoxicosis (type II).

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Documento generato il 09/04/20 alle ore 06:54:26