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Titolo:
Expression of adhesion molecules on CD34(+) cells in peripheral blood of non-Hodgkin's lymphoma patients mobilized with different growth factors
Autore:
Gazitt, Y; Shaughnessy, P; Liu, Q;
Indirizzi:
Univ Texas, Hlth Sci Ctr, Dept Med Hematol, San Antonio, TX 78284 USA UnivTexas San Antonio TX USA 78284 ed Hematol, San Antonio, TX 78284 USA Wilford Hall USAF Med Ctr, San Antonio, TX 78236 USA Wilford Hall USAF MedCtr San Antonio TX USA 78236 Antonio, TX 78236 USA
Titolo Testata:
STEM CELLS
fascicolo: 2, volume: 19, anno: 2001,
pagine: 134 - 143
SICI:
1066-5099(2001)19:2<134:EOAMOC>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; HEMATOPOIETIC PROGENITOR CELLS; FACTOR G-CSF; HIGH-DOSE CHEMOTHERAPY; CLONAL MYELOMA CELLS; HUMAN FETAL LIVER; STEM-CELLS; BONE-MARROW; PLATELET RECOVERY; ENGRAFTMENT KINETICS;
Keywords:
mobilization; CD34(+); VLA-4; L-selectin; NHL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Gazitt, Y Univ Texas, Hlth Sci Ctr, Dept Med Hematol, 7703 Floyd Curl Dr, San Antonio, TX 78284 USA Univ Texas 7703 Floyd Curl Dr San Antonio TX USA 78284 78284 USA
Citazione:
Y. Gazitt et al., "Expression of adhesion molecules on CD34(+) cells in peripheral blood of non-Hodgkin's lymphoma patients mobilized with different growth factors", STEM CELLS, 19(2), 2001, pp. 134-143

Abstract

Adhesion molecules on CD34(+) cells were implicated in the process of peripheral blood stem cell (PBSC) mobilization and homing. We studied the mobilization of CD34(+)Thy1(+) cells, CD34(+) very late-acting antigen (VLA)4(+)cells, and CD34(+)L-selectin(+) cells in non-Hodgkin's lymphoma patients mobilized with cyclophosphamide plus G-CSF, GM-CSF, or GM-CSF followed by G-CSF. The mean percentage of CD34(+) cells in the bone marrow (BM) expressing Thy1 was 23.6% +/- 11% and 17.8% +/- 8% in the PB before mobilization, and was markedly decreased to 4.5% +/- 3.3% in the apheresis collections. Similarly, the mean percentage of CD34(+) cells expressing L-selectin was 35.8% +/- 4.3% in the BM, 21.6% +/- 4.1% in the PB before mobilization and was markedly decreased to 9.1% +/- 2.5% in the apheresis collections. Patients in the three arms of the study had a similar pattern of CD34(+)Thy1(+) and CD34(+)L-selectin(+) cell mobilization. Also, a similar pattern of coexpression of CD34(+)Thy1(+) and CD34(+)L-selectin(+) cells was observed when thepatients were regrouped as "good mobilizers" (greater than or equal to2 x 10(6) CD34(+)CD45(dim) cells/kg, in four collections) and "poor mobilizers"(<0.4 x 10(6) CD34(+)CD45(dim) cells/kg, in two collections). The mean percentage of CD34(+) cells expressing VLA-4 in the BM and PB wasrelatively high (73.4% +/- 12% and 65.4% +/- 6.6%, respectively) and dropped considerably in the PBSC collections to 43.5% +/- 7.1% with a similar pattern observed for patients in arms A, B, and C. However, when the patientswere regrouped as "good mobilizers" and "poor mobilizers," a higher percentage of CD34(+) cells expressing VLA-4 was observed in the PBSC of the pooled "good mobilizers" (50.5% +/- 9% versus 36.3% +/- 6.4%; p = 0.01). We conclude that release of CD34(+) cells to the PB involves a general downregulation of Thy1, L-selectin and VLA-4 on CD34(+) cells, irrespective of the growth factor used for mobilization. However, good mobilizers had a relatively higher percentage of CD34(+) cells expressing the VLA-4 antigen.

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Documento generato il 21/09/20 alle ore 14:53:58