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Titolo:
"Rainbow" reporters for multispectral marking and lineage analysis of hematopoietic stem cells
Autore:
Hawley, TS; Telford, WG; Hawley, RG;
Indirizzi:
Amer Red Cross, Jerome H Holland Lab Biomed Sci, Dept Hematopoiesis, Rockville, MD 20855 USA Amer Red Cross Rockville MD USA 20855 atopoiesis, Rockville, MD 20855 USA Amer Red Cross, Jerome H Holland Lab Biomed Sci, Flow Cytometry Facil, Rockville, MD 20855 USA Amer Red Cross Rockville MD USA 20855 etry Facil, Rockville, MD 20855 USA NCI, Flow Cytometry Core Lab, Med Branch, NIH, Bethesda, MD 20892 USA NCIBethesda MD USA 20892 re Lab, Med Branch, NIH, Bethesda, MD 20892 USA George Washington Univ, Med Ctr, Dept Anat & Cell Biol, Washington, DC 20037 USA George Washington Univ Washington DC USA 20037 , Washington, DC 20037 USA
Titolo Testata:
STEM CELLS
fascicolo: 2, volume: 19, anno: 2001,
pagine: 118 - 124
SICI:
1066-5099(2001)19:2<118:"RFMMA>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
GREEN FLUORESCENT PROTEIN; GENE-TRANSFER; FLOW-CYTOMETRY; LENTIVIRAL VECTORS; EXPRESSION; THERAPY; GFP; EXCITATION; VARIANTS; COLOR;
Keywords:
fluorescent protein reporter genes; multiparameter flow cytometry; oncoretroviral and lentiviral vectors; gene transfer; hematopoietic stem cells;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Hawley, RG Amer Red Cross, Jerome H Holland Lab Biomed Sci, Dept Hematopoiesis, 15601Crabbs Branch Way, Rockville, MD 20855 USA Amer Red Cross 15601 Crabbs Branch Way Rockville MD USA 20855 A
Citazione:
T.S. Hawley et al., ""Rainbow" reporters for multispectral marking and lineage analysis of hematopoietic stem cells", STEM CELLS, 19(2), 2001, pp. 118-124

Abstract

Hematologic diseases potentially benefiting from gene-based therapies involving hematopoietic stem cells (HSCs) include hereditary hemoglobinopathies, immunodeficiency syndromes, and congenital bleeding disorders such as hemophilia A, as well as acquired diseases like AIDS, Successful treatment of these blood diseases with gene-modified HSCs requires high efficiency gene delivery to the target cell population and persistence of transgene expression following differentiation. We review flow cytometric procedures that permit simultaneous, noninvasive measurements of transgene expression and phenotypic discrimination of hematopoietic cell subsets. Central to this approach has been the recent development of a spectrum of blue, cyan, and yellowish-green fluorescent reporters based on the jellyfish Aequorea victor ia green fluorescent protein and the discovery of a red fluorescent protein in Discosoma coral. This methodology should facilitate the optimization of oncoretroviral and lentiviral vectorology and HSC transduction protocols for the ultimate purpose of HSC-directed gene therapy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 12:28:32