Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
In vitro assembly of phytochrome B apoprotein with synthetic analogs of the phytochrome chromophore
Autore:
Hanzawa, H; Inomata, K; Kinoshita, H; Kakiuchi, T; Jayasundera, KP; Sawamoto, D; Ohta, A; Uchida, K; Wada, K; Furuya, M;
Indirizzi:
Hitachi Adv Res Lab, Hatoyama, Saitama 3500395, Japan Hitachi Adv Res LabHatoyama Saitama Japan 3500395 Saitama 3500395, Japan Kanazawa Univ, Grad Sch Nat Sci & Technol, Kanazawa, Ishikawa 9201192, Japan Kanazawa Univ Kanazawa Ishikawa Japan 9201192 wa, Ishikawa 9201192, Japan
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 6, volume: 98, anno: 2001,
pagine: 3612 - 3617
SICI:
0027-8424(20010313)98:6<3612:IVAOPB>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECOMBINANT PHYTOCHROME; ARABIDOPSIS-THALIANA; ANGSTROM RESOLUTION; A,B-RINGS COMPONENT; SPECTRAL PROPERTIES; DIMETHYL ESTER; WILD-TYPE; PHYCOCYANOBILIN; APOPHYTOCHROME; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Furuya, M Hitachi Adv Res Lab, Hatoyama, Saitama 3500395, Japan Hitachi Adv Res Lab Hatoyama Saitama Japan 3500395 00395, Japan
Citazione:
H. Hanzawa et al., "In vitro assembly of phytochrome B apoprotein with synthetic analogs of the phytochrome chromophore", P NAS US, 98(6), 2001, pp. 3612-3617

Abstract

Phytochrome B (PhyB), one of the major photosensory chromoproteins in plants, mediates a variety of light-responsive developmental processes in a photoreversible manner. To analyze the structural requirements of the chromophore for the spectral properties of PhyB, we have designed and chemically synthesized 20 analogs of the linear tetrapyrrole (bilin) chromophore and reconstituted them with PhyB apoprotein (PHYB). The A-ring acts mainly as the anchor for ligation to PHYB, because the modification of the side chains atthe C2 and C3 positions did not significantly influence the formation or difference spectra of adducts. In contrast, the side chains of the B- and C-rings are crucial to position the chromophore properly in the chromophore pocket of PHYB and for photoreversible spectral changes. The side-chain structure of the D-ring is required for the photoreversible spectral change of the adducts. When methyl and ethyl groups at the C17 and C18 positions are replaced with an n-propyl, n-pentyl, or n-octyl group, respectively, the photoreversible spectral change of the adducts depends on the length of the side chains. From these studies, we conclude that each pyrrole ring of the linear tetrapyrrole chromophore plays a different role in chromophore assembly and the photochromic properties of PhyB.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 19:25:12