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Titolo:
Enhancement of matrix metalloproteinase (MMP)-2 activity in gingival tissue and cultured fibroblasts from Down's syndrome patients
Autore:
Komatsu, T; Kubota, E; Sakai, N;
Indirizzi:
Kanagawa Dent Coll, Dept Oral & Maxillofacial Surg, Yokosuka, Kanagawa 2380003, Japan Kanagawa Dent Coll Yokosuka Kanagawa Japan 2380003 anagawa 2380003, Japan Kanagawa Dent Coll, Dept Dent Special Patients, Yokosuka, Kanagawa 2380003, Japan Kanagawa Dent Coll Yokosuka Kanagawa Japan 2380003 anagawa 2380003, Japan
Titolo Testata:
ORAL DISEASES
fascicolo: 1, volume: 7, anno: 2001,
pagine: 47 - 55
SICI:
1354-523X(200101)7:1<47:EOMM(A>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN PERIODONTAL-DISEASES; IV COLLAGENASE; INTERSTITIAL COLLAGENASES; CREVICULAR FLUID; TETRACYCLINE-INHIBITION; JUVENILE PERIODONTITIS; PROGELATINASE-A; EXPRESSION; ACTIVATION; 72-KDA;
Keywords:
Down's syndrome; gingival fibroblasts; matrix metalloproteinase; gingivitis; periodontitis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Kubota, E Kanagawa Dent Coll, Dept Oral & Maxillofacial Surg, 82 Inaoka Cho, Yokosuka, Kanagawa 2380003, Japan Kanagawa Dent Coll 82 Inaoka Cho Yokosuka Kanagawa Japan 2380003
Citazione:
T. Komatsu et al., "Enhancement of matrix metalloproteinase (MMP)-2 activity in gingival tissue and cultured fibroblasts from Down's syndrome patients", ORAL DIS, 7(1), 2001, pp. 47-55

Abstract

OBJECTIVES: To identify one of the possible factors responsible for periodontal disease in Down's syndrome (trisomy 21) patients, we studied the enzyme activity and the mRNA expression pattern of matrix metalloproteinases (MMPs) of cultured gingival fibroblasts (GF) and fresh gingival tissues. MATERIALS AND METHODS: Gingival tissue was used as the cell source and wasbiopsied at the time of dental treatment from nine patients with Down's syndrome and nine non-Down's controls. GF were cultivated in serum-free mediafor analyses of their MMP activities at the transcription or the protein level. The MMP activities in the supernates were measured by gelatin impregnated zymography. Relative levels of MMP mRNA from the cultured GF or freshly isolated gingival tissues were determined using the reverse transcriptionpolymerase chain reaction (RT-PCR). RESULT AND CONCLUSIONS: The production of the active type of MMP-2 in GF from Down's syndrome patients (D-GF) was found to be significantly higher (P< 0.05) than that of the control GF (C-GF) at the protein level. The mRNA expressions of membrane-typeI MMP (MTI-MMP) and MMP-2 in D-GF were constitutively augmented when compared with those of C-GF. These findings suggest that specific increase of the active form of MMP-2 in D-GF may possibly be due to the concomitant expression of MTI-MMP in the cultured cells, and thiscould be related to the pathogenesis of gingivitis/periodontitis associated with Down's syndrome patients.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 09:40:03