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Titolo:
E-cadherin gene (CDH1) promoter methylation as the second hit in sporadic diffuse gastric carcinoma
Autore:
Machado, JC; Oliveira, C; Carvalho, R; Soares, P; Berx, G; Caldas, C; Seruca, R; Carneiro, F; Sobrinho-Simoes, M;
Indirizzi:
IPATIMUP, P-4200 Oporto, Portugal IPATIMUP Oporto Portugal P-4200IPATIMUP, P-4200 Oporto, Portugal Univ Cambridge, Dept Oncol, Cambridge CB2 2XY, England Univ Cambridge Cambridge England CB2 2XY col, Cambridge CB2 2XY, England VIB Ghent Univ, KL, Dept Mol Biol, B-9000 Ghent, Belgium VIB Ghent Univ Ghent Belgium B-9000 Dept Mol Biol, B-9000 Ghent, Belgium Univ Porto, Fac Med, P-4200 Oporto, Portugal Univ Porto Oporto Portugal P-4200 orto, Fac Med, P-4200 Oporto, Portugal
Titolo Testata:
ONCOGENE
fascicolo: 12, volume: 20, anno: 2001,
pagine: 1525 - 1528
SICI:
0950-9232(20010322)20:12<1525:EG(PMA>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOBULAR BREAST CANCERS; MUTATIONS PROVIDE; EXPRESSION; HYPERMETHYLATION; CELLS; PROGRESSION; PATTERNS; ADHESION; ISLAND;
Keywords:
E-cadherin; mutation; methylation; gastric cancer; stomach; diffuse cancer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Machado, JC IPATIMUP, Rua Roberto Frias S-N, P-4200 Oporto, Portugal IPATIMUP Rua Roberto Frias S-N Oporto Portugal P-4200 ortugal
Citazione:
J.C. Machado et al., "E-cadherin gene (CDH1) promoter methylation as the second hit in sporadic diffuse gastric carcinoma", ONCOGENE, 20(12), 2001, pp. 1525-1528

Abstract

In diffuse gastric carcinoma, despite common E-cadherin gene (CDH1) mutations, tumors show absence of CDH1 loss of heterozigosity (LOH) in most cases. This observation challenges the classical two-hit model of tumor suppresser gene inactivation. In order to investigate whether or not CDH1 promoter methylation may function as the second hit we analysed a series of 23 sporadic gastric carcinomas for the presence of CDH1 mutations, CDH1 promoter methylation, LOH and E-cadherin expression. CDH1 mutations were detected in nine of the 16 (56.3%) diffuse gastric carcinomas and in none of the seven intestinal gastric carcinomas. In diffuse gastric carcinomas harboring CDH1 mutations, LOH was observed in a single case. Loss of plasma membrane E-cadherin expression was consistently found in all nine cases with CDH1 mutation, suggesting that tumors inactivated the remaining CDH1 allele via a different mechanism. CDH1 promoter methylation was observed in nine of the 16 (56.3%) diffuse-type gastric carcinoma cases, including six of the nine cases(66.7%) harboring CDH1 mutations. CDH1 promoter methylation was also seen in two (28.6%) intestinal-type cases. Our results show that CDH1 promoter methylation is the second hit in more than half of the sporadic diffuse gastric carcinoma cases harboring CDH1 mutations.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 12:45:18