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Titolo:
Src family kinases and HER2 interactions in human breast cancer cell growth and survival
Autore:
Belsches-Jablonski, AP; Biscardi, JS; Peavy, DR; Tice, DA; Romney, DA; Parsons, SJ;
Indirizzi:
Univ Virginia, Hlth Sci Ctr, Dept Microbiol, Charlottesville, VA 22908 USAUniv Virginia Charlottesville VA USA 22908 Charlottesville, VA 22908 USA Univ Virginia, Hlth Sci Ctr, Ctr Canc, Charlottesville, VA 22908 USA Univ Virginia Charlottesville VA USA 22908 Charlottesville, VA 22908 USA Lynchburg Coll, Biol Program, Lynchburg, VA 24501 USA Lynchburg Coll Lynchburg VA USA 24501 ol Program, Lynchburg, VA 24501 USA
Titolo Testata:
ONCOGENE
fascicolo: 12, volume: 20, anno: 2001,
pagine: 1465 - 1475
SICI:
0950-9232(20010322)20:12<1465:SFKAHI>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-TYROSINE KINASES; ERBB SIGNALING NETWORK; FACTOR RECEPTOR; C-SRC; PHOSPHATIDYLINOSITOL 3-KINASE; DIFFERENTIATION FACTOR; NEU PROTOONCOGENE; EPITHELIAL-CELLS; OVARIAN-CANCER; A431 CELLS;
Keywords:
Src family kinases; heregulin; breast cancer; HER2/neu; cell growth; apoptosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
63
Recensione:
Indirizzi per estratti:
Indirizzo: Parsons, SJ Univ Virginia, Hlth Sci Ctr, Dept Microbiol, Box 441, Charlottesville, VA 22908 USA Univ Virginia Box 441 Charlottesville VA USA 22908 A 22908 USA
Citazione:
A.P. Belsches-Jablonski et al., "Src family kinases and HER2 interactions in human breast cancer cell growth and survival", ONCOGENE, 20(12), 2001, pp. 1465-1475

Abstract

Evidence from murine fibroblast models and human breast cancer cells indicates that c-Src and human EGF receptor (HER1) synergize to enhance neoplastic growth of mammary epithelial cells, To investigate whether interactions between c-Src and other HER members may also play a role in breast tumor progression, we characterized 13 human breast carcinoma cell lines and 13 tumor samples for expression of HER family members and c-Src and examined a subset of the cell lines for Src-dependent, heregulin (HRG)-augmented, anchorage-dependent and independent growth. By immunoblotting, we found that all cell lines overexpressed one or more HER family member, and 60% overexpressed c-Src, Seventy-five per cent of the tumor tissues overexpressed HER2, while 64% overexpressed c-Src, Colony formation in soft agar was enhanced by HRG in three of five cell lines tested, a response that correlated,vith thepresence of a c-Src/HER2 heterocomplex, This result suggests that HRG may act through both HER2 and c-Src to facilitate anchorage-independent growth. In contrast, HRG had little effect on anchorage-dependent growth in any ofthe cell lines tested. PP1, a Src family kinase inhibitor, reduced or ablated HRG-dependent and independent soft agar growth or anchorage dependent growth, and triggered apoptosis in all cell lines tested. The apoptotic effect of PP1 could be partially or completely reversed by HRG, depending on the cell line. These results suggest that while Src family kinases may cooperate with HRG to promote the survival and growth of human breast tumor cells, they also function independently of HER2/HRG in these processes.

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Documento generato il 03/07/20 alle ore 22:56:56