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Titolo:
NGF enhances depolarization effects on SNAP-25 expression: induction of SNAP-25b isoform
Autore:
Hepp, R; Dupont, JL; Aunis, D; Langley, K; Grant, NJ;
Indirizzi:
INSERM, Unite 338, F-67084 Strasbourg, France INSERM Strasbourg France F-67084 , Unite 338, F-67084 Strasbourg, France CNRS, FRE 2180, F-67084 Strasbourg, France CNRS Strasbourg France F-67084 NRS, FRE 2180, F-67084 Strasbourg, France
Titolo Testata:
NEUROREPORT
fascicolo: 4, volume: 12, anno: 2001,
pagine: 673 - 677
SICI:
0959-4965(20010326)12:4<673:NEDEOS>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
TYROSINE-HYDROXYLASE GENE; DIFFERENTIAL EXPRESSION; PROTEIN SNAP-25; ADRENAL-GLAND; PC12 CELLS; RAT-BRAIN; CALCIUM; IDENTIFICATION; INVOLVEMENT; PLASTICITY;
Keywords:
CNS development; depolarization; neuroplasticity; neurosecretion; PC12 cells; SNAP-25 isoforms;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Grant, NJ INSERM, Unite 338, 5 Rue Blaise Pascal, F-67084 Strasbourg, France INSERM 5 Rue Blaise Pascal Strasbourg France F-67084 rg, France
Citazione:
R. Hepp et al., "NGF enhances depolarization effects on SNAP-25 expression: induction of SNAP-25b isoform", NEUROREPORT, 12(4), 2001, pp. 673-677

Abstract

The 25 kDa synaptosomal associated protein (SNAP-25), which is implicated in neuronal plasticity and neurosecretion, exists as two isoforms generatedby alternative splicing of exons 5a and 5b. The aim of the present study was to characterize factors influencing isoform expression. We report that chronic depolarization of PC12 cells alone or in the presence of NGF inducesthe expression of isoform-b, in addition to a 1.8- to 3-fold increase in SNAP-25 mRNA and protein as determined by immunoblotting and combined RT-PCRand Southern blot analysis. When cerebellar granule neurons were cultured in elevated K+, the predominant isoform switched from SNAP-25a to SNAP-25b. Taken together these results suggested that chronic depolarization regulates the transcription and processing of SNAP-25 mRNA. NeuroReport 12:673-677(C) 2001 Lippincott Williams & Wilkins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 13:24:27