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Titolo:
Effects of short- and long-term exposure to c-AMP and c-GMP on the noradrenaline transporter
Autore:
Bryan-Lluka, LJ; Paczkowski, FA; Bonisch, H;
Indirizzi:
Univ Queensland, Dept Physiol & Pharmacol, Brisbane, Qld 4072, Australia Univ Queensland Brisbane Qld Australia 4072 Brisbane, Qld 4072, Australia Univ Bonn, Dept Pharmacol & Toxicol, D-53113 Bonn, Germany Univ Bonn Bonn Germany D-53113 harmacol & Toxicol, D-53113 Bonn, Germany
Titolo Testata:
NEUROPHARMACOLOGY
fascicolo: 4, volume: 40, anno: 2001,
pagine: 607 - 617
SICI:
0028-3908(200103)40:4<607:EOSALE>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; HUMAN SEROTONIN TRANSPORTER; HUMAN DOPAMINE TRANSPORTER; N-SH CELLS; NOREPINEPHRINE TRANSPORTER; DIFFERENTIAL REGULATION; PHEOCHROMOCYTOMA CELLS; CHORIOCARCINOMA CELLS; SURFACE EXPRESSION; PHORBOL ESTERS;
Keywords:
noradrenaline transporter; c-AMP; c-GMP; regulation; rat PC12 cells; human SK-N-SH-SY5Y cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Bryan-Lluka, LJ Univ Queensland, Dept Physiol & Pharmacol, Brisbane, Qld 4072, Australia Univ Queensland Brisbane Qld Australia 4072 72, Australia
Citazione:
L.J. Bryan-Lluka et al., "Effects of short- and long-term exposure to c-AMP and c-GMP on the noradrenaline transporter", NEUROPHARM, 40(4), 2001, pp. 607-617

Abstract

The effects of short- and long-term exposure of cells to elevated cyclic adenosine monophosphate (c-AMP), using dibutyryl-c-AMP, 8-bromo-c-AMP, cholera toxin or forskolin, or cyclic guanosine monophosphate (c-GMP), using dibutyryl-c-GMP or 8-bromo-c-GMP, on the activity and expression of the noradrenaline transporter (NAT) were examined. Short- or long-term c-GMP elevation had no effects on H-3-noradrenaline uptake by rat PC12 phaeochromocytoma cells or human SK-N-SH-SY5Y neuroblastoma cells. Short-term c-AMP elevation(for 17 min experiment duration) caused a decrease in H-3-noradrenaline uptake by PC12 cells, but had no effects on SK-N-SH-SY5Y cells or COS-7 cellstransfected with human or rat NAT cDNA. c-AMP did not affect H-3-nisoxetine binding to PC12 cells. Long-term (24 h) exposure to elevated c-AMP levelscaused a decrease in H-3-noradrenaline uptake and NAT mRNA in PC12 cells, but had no effects on SK-N-SH-SY5Y cells and caused a small increase in H-3-noradrenaline uptake in COS-7 cells heterologously expressing rat or humanNAT. Hence, c-AMP, but not c-GMP, causes a cell type-dependent reduction in NAT activity after short-term exposure and a reduction in NAT expression after long-term exposure. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/07/20 alle ore 08:04:34