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Titolo:
Erythropoietin modulates angiotensin II- or noradrenaline-induced Ca2+ mobilization in cultured rat vascular smooth-muscle cells
Autore:
Akimoto, T; Kusano, E; Fujita, N; Okada, K; Saito, O; Ono, S; Ando, Y; Homma, S; Saito, T; Asano, Y;
Indirizzi:
Jichi Med Sch, Dept Nephrol, Minamikawachi, Tochigi 3290498, Japan Jichi Med Sch Minamikawachi Tochigi Japan 3290498 Tochigi 3290498, Japan Jichi Med Sch, Dept Endocrinol & Metab, Minamikawachi, Tochigi 3290498, Japan Jichi Med Sch Minamikawachi Tochigi Japan 3290498 Tochigi 3290498, Japan
Titolo Testata:
NEPHROLOGY DIALYSIS TRANSPLANTATION
fascicolo: 3, volume: 16, anno: 2001,
pagine: 491 - 499
SICI:
0931-0509(200103)16:3<491:EMAION>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECOMBINANT-HUMAN-ERYTHROPOIETIN; FREE CALCIUM-CONCENTRATION; CYTOSOLIC-FREE CALCIUM; PROTEIN-KINASE-C; INTERLEUKIN-1-BETA-STIMULATED NITRIC-OXIDE; SPONTANEOUSLY HYPERTENSIVE RATS; HEMODIALYSIS-PATIENTS; BLOOD-PRESSURE; RENAL-FAILURE; HUMAN ERYTHROBLASTS;
Keywords:
angiotensin II; cytosolic free Ca2+ mobilization; erythropoietin; noradrenaline; protein kinase C; vascular smooth-muscle cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Kusano, E Jichi Med Sch, Dept Nephrol, Yakushiji 3311-1, Minamikawachi, Tochigi 3290498, Japan Jichi Med Sch Yakushiji 3311-1 Minamikawachi Tochigi Japan 3290498
Citazione:
T. Akimoto et al., "Erythropoietin modulates angiotensin II- or noradrenaline-induced Ca2+ mobilization in cultured rat vascular smooth-muscle cells", NEPH DIAL T, 16(3), 2001, pp. 491-499

Abstract

Background. It has been reported that human recombinant erythropoietin (rHuEpo) modulates the sensitivity of the cardiovascular system to angiotensinII (Ang II) or noradrenaline (NA). In the present study, we explored the effect of rHuEpo on the responsiveness of Ang II- or NA-induced cytosolic free calcium ([Ca2+]i) mobilization in cultured rat vascular smooth-muscle cells (VSMC). Methods.[Ca2+]i concentrations in VSMC were measured by using the calcium-sensitive fluorescent dye fura-2. Results. The addition of rHuEpo (250 U/ml) alone induced elevation in [Ca2]i, which remained significantly elevated above basal level for at least 60 min in the presence of extracellular Ca2+. Pre-incubation with specific protein kinase C (PKC) inhibitor calphostin C (1 mu mol/l) significantly reduced the peak and the sustained elevations of [Ca2+]i. Pre-treatment with rHuEpo for 60 min increased both basal [Ca2+]i and the changes in [Ca2+]i byAng II or NA in a dose-dependent manner in the presence of extracellular Ca2+. The synergistic effects of rHuEpo with Ang II or NA were also retainedwhen VSMC were bathed in the Ca2+-free medium after the pre-incubation of rHuEpo. Conversely, they were diminished in the presence of extracellular Ca2+ combined with intracellular Ca2+ release inhibitor 8-(NN-diethylamino)octyl-1,3,4,5-trimethoxybenzoate (TMB-8). The synergistic effects of rHuEpo were also diminished by PKC depletion or by PKC inhibitor. Conclusions. These observations suggest that rHuEpo has synergistic effects on Ang II- or NA-induced [Ca2+]i mobilization, particularly on intracellular Ca2+ release, in VSMC. This may be a potential mechanism contributing to hypertension associated with rHuEpo therapy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 00:12:32