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Titolo:
Dopamine receptors in human platelets
Autore:
Ricci, A; Bronzetti, E; Mannino, F; Mignini, F; Morosetti, C; Tayebati, SK; Amenta, F;
Indirizzi:
Univ Camerino, Dipartimento Sci Farmacol & Med Sperimentale, Sez Anat Umana, I-62032 Camerino, Italy Univ Camerino Camerino Italy I-62032 Anat Umana, I-62032 Camerino, Italy Univ La Sapienza, Dipartimento Sci Cardiovasc & Resp, Rome, Italy Univ La Sapienza Rome Italy rtimento Sci Cardiovasc & Resp, Rome, Italy Unita Operat Anat Patol, Jesi, Italy Unita Operat Anat Patol Jesi ItalyUnita Operat Anat Patol, Jesi, Italy
Titolo Testata:
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
fascicolo: 4, volume: 363, anno: 2001,
pagine: 376 - 382
SICI:
0028-1298(200104)363:4<376:DRIHP>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-BLOOD PLATELETS; BINDING; AGONISTS; 5-HYDROXYTRYPTAMINE; IDENTIFICATION; VASCULATURE; DISEASE; PROTEIN; RABBIT; SITES;
Keywords:
dopamine receptors; platelets; anti-receptor antibodies; Western blot; immunocytochemistry; aggregation; human;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Amenta, F Univ Camerino, Dipartimento Sci Farmacol & Med Sperimentale, SezAnat Umana, Via Scalzino 5, I-62032 Camerino, Italy Univ Camerino Via Scalzino 5 Camerino Italy I-62032 rino, Italy
Citazione:
A. Ricci et al., "Dopamine receptors in human platelets", N-S ARCH PH, 363(4), 2001, pp. 376-382

Abstract

The expression of dopamine receptors by human platelets was investigated by Western blot analysis and immunocytochemical techniques using antibodies raised against dopamine D1-D5 receptor protein. The influence of dopamine D1-like and D2-like receptor agonists on adrenaline-induced platelet aggregation was also investigated. Western blot analysis revealed that platelet membranes bind anti-dopamine D3 or D5 receptor protein antibodies, but not anti-D1, D2 or D4 receptor protein antibodies. Cytospin centrifuged human platelets exposed to anti-dopamine D3 or D5 receptor protein antibodies developed a specific immune staining, whereas no positive staining was noticeablein platelets exposed to other antibodies tested. Both the D1-like receptoragonist 1-phenyl-2,3,4,5-tetrahydro-(1H)-3-benzazepine-7, hydrochloride (SKF 38393) and the D2-like receptor agonist 7-hydroxy-N,N-di-n-propyl-2-aminotetralin (7-OH-DPAT) dose-dependently inhibited adrenaline-induced platelet aggregation. These effects were decreased respectively by the D1-like andD2-like receptor antagonists R(+)-2,3,4,5-tetrahydro- 3-methyl-5-phenyl-1H-3-benzazepin-7-ol hydrochloride (SCH 23390) and (-)sulpiride. The above findings indicate that human platelets express dopamine D3 and D5 receptors probably involved in the regulation of platelet function.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 06:41:33