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Titolo:
Evidence for an endocannabinoid system in the central nervous system of the leech Hirudo medicinalis
Autore:
Matias, I; Bisogno, T; Melck, D; Vandenbulcke, F; Verger-Bocquet, M; De Petrocellis, L; Sergheraert, C; Breton, C; Di Marzo, V; Salzet, M;
Indirizzi:
Univ Sci & Tech Lille Flandres Artois, Lab Endocrinol & Immunite Annelides, CNRS, UPRESA 8017,SN3, F-59655 Villeneuve Dascq, France Univ Sci & Tech Lille Flandres Artois Villeneuve Dascq France F-59655 ce CNR, Ist Chim Mol Interesse Biol, Endocannabinoid Res Grp, I-80072 Arco, Napoli, Italy CNR Arco Napoli Italy I-80072 binoid Res Grp, I-80072 Arco, Napoli, Italy CNR, Ist Cibernet, I-80072 Arco, Napoli, Italy CNR Arco Napoli Italy I-80072 , Ist Cibernet, I-80072 Arco, Napoli, Italy Inst Biol Lille, CNRS, UMR 8525, Lab Synth Struct & Fonct Biomol, F-59021 Lille, France Inst Biol Lille Lille France F-59021 Fonct Biomol, F-59021 Lille, France
Titolo Testata:
MOLECULAR BRAIN RESEARCH
fascicolo: 2, volume: 87, anno: 2001,
pagine: 145 - 159
SICI:
0169-328X(20010305)87:2<145:EFAESI>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACID AMIDE HYDROLASE; CANNABINOID RECEPTOR AGONIST; NITRIC-OXIDE RELEASE; MOLECULAR CHARACTERIZATION; ANANDAMIDE AMIDOHYDROLASE; N-ACYLETHANOLAMINES; CB1 RECEPTOR; BRAIN; IDENTIFICATION; NEURONS;
Keywords:
leech; central nervous system; endocannabinoid; anandamide amidase; cannabinoid receptor; localization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Salzet, M Univ Sci & Tech Lille Flandres Artois, Lab Endocrinol & ImmuniteAnnelides, CNRS, UPRESA 8017,SN3, F-59655 Villeneuve Dascq, France Univ Sci & Tech Lille Flandres Artois Villeneuve Dascq France F-59655
Citazione:
I. Matias et al., "Evidence for an endocannabinoid system in the central nervous system of the leech Hirudo medicinalis", MOL BRAIN R, 87(2), 2001, pp. 145-159

Abstract

In invertebrates, like Hydra and sea urchins, evidence for a functional cannabinoid system was described. The partial characterization of a putative CB1 cannabinoid receptor in the leech Hirudo medicinalis led us to investigate the presence of a complete endogenous cannabinoid system in this organism. By using gas chromatography-mass spectrometry, we demonstrate the presence of the endocannabinoids anandamide (N-arachidonoylethanolamine, 21.5+/-0.7 pmol/g) and 2-arachidonoyl-glycerol (147.4+/-42.7 pmol/g), and of the biosynthetic precursor of anandamide, N-arachidonylphosphatidyl-ethanolamine(16.5+/-3.3 pmol/g), in the leech central nervous system (CNS). Anandamide-related molecules such as N-palmitoylethanolamine (32.4+/-1.6 pmol/g) and N-linolenoylethanolamine (5.8 pmol/g) were also detected. We also found an anandamide amidase activity in the leech CNS cytosolic fraction with a maximal activity at pH 7 and little sensitivity to typical fatty acid amide hydrolase (FAAH) inhibitors. Using an antiserum directed against the amidase signature sequence, we focused on the identification and the localization ofthe leech amidase. Firstly, leech nervous system protein extract was subjected to Western blot analysis, which showed three immunoreactive bands at ca. similar to 42, similar to 46 and similar to 66 kDa. The former and latter bands were very faint and were also detected in whole homogenates from the coelenterate Hydra vulgaris, where the presence of CB1-like receptors, endocannabinoids and a FAAH-like activity was reported previously. Secondly, amidase immunocytochemical detection revealed numerous immunoreactive neurons in the CNS of three species of leeches. In addition, we observed that leech amidase-like immunoreactivity matches to a certain extent with CB1-likeimmunoreactivity. Finally, we also found that stimulation by anandamide ofthis receptor leads, as in mammals, to inhibition of cAMP formation, although this effect appeared to be occurring through the previously described anandamide-induced and CB1-mediated activation of nitric oxide release. Taken together, these results suggest the existence of a complete and functional cannabinoid system in leeches. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 23:16:59