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Titolo:
Re-evaluation of nestin as a marker of oligodendrocyte lineage cells
Autore:
Almazan, G; Vela, JM; Molina-Holgado, E; Guaza, C;
Indirizzi:
McGill Univ, Dept Pharmacol & Therapeut, Montreal, PQ H3G 1Y6, Canada McGill Univ Montreal PQ Canada H3G 1Y6 peut, Montreal, PQ H3G 1Y6, Canada Univ Autonoma Barcelona, Fac Med, Histol Unit, Bellaterra 08193, Barcelona, Spain Univ Autonoma Barcelona Bellaterra Barcelona Spain 08193 Barcelona, Spain CSIC, Inst Cajal, Neural Plast Unit, E-28002 Madrid, Spain CSIC Madrid Spain E-28002 ajal, Neural Plast Unit, E-28002 Madrid, Spain
Titolo Testata:
MICROSCOPY RESEARCH AND TECHNIQUE
fascicolo: 6, volume: 52, anno: 2001,
pagine: 753 - 765
SICI:
1059-910X(20010315)52:6<753:RONAAM>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; GLIAL PROGENITOR-CELL; DEVELOPING RAT-BRAIN; PDGF ALPHA-RECEPTOR; STEM-CELLS; MONOCLONAL-ANTIBODY; CEREBRAL-CORTEX; PRECURSOR CELLS; GROWTH-FACTOR; SUBEPENDYMAL CELLS;
Keywords:
nestin; intermediate filament; oligodendrocyte progenitors; pre-O2A-progenitors; myelination; glial cell lineage;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
68
Recensione:
Indirizzi per estratti:
Indirizzo: Almazan, G McGill Univ, Dept Pharmacol & Therapeut, 3655 Drummond St, Montreal, PQ H3G 1Y6, Canada McGill Univ 3655 Drummond St Montreal PQ Canada H3G 1Y6 Canada
Citazione:
G. Almazan et al., "Re-evaluation of nestin as a marker of oligodendrocyte lineage cells", MICROSC RES, 52(6), 2001, pp. 753-765

Abstract

Maturation of oligodendrocyte progenitors (O2A) is characterized by morphological changes and the sequential expression of specific antigens leading to the formation of myelin membrane. Monoclonal antibodies A2B5, A007, anti-vimentin, and anti-galactocerebroside, recognize oligodendroglia at different stages of development. The neuroepithelial precursor marker nestin is also expressed by the oligodendroglial lineage; we have used enriched populations of progenitors isolated from neonatal rat brain cultures to further examine the cellular distribution of this intermediate filament protein. Thephenotypic distribution of nestin positive cells among the oligodendrocyteLineage showed that 65% reacted with A2B5, whereas only 5% were A007(+), and 4% galactocerebroside(+)'. The remaining 25% of the cells were not labeled and had small cellular bodies devoid of processes, characteristic of thepre-O2A progenitor. Further analysis of the nestin(+) population showed that the majority of the cells were also vimentin(+). Antibody-dependent complement mediated cytolysis of A2B5(+) (O2A cells) and galactocerebroside(+) (mature oligodendrocytes) cells left a population of nestin(+) cells that were induced to proliferate in the presence of growth factors and to differentiate into A2B5(+) and galactocerebroside(+) cells. Proliferating cells maintained in the presence of platelet-derived growth factor or basic fibroblast growth factor retained nestin expression along with A2B5. By contrast, in serum-free medium nestin expression decreased while postmitotic cells acquired A007 and galactocerebroside. Our results suggest that nestin expression is a marker of pre-O2A cells that is maintained in proliferating glial progenitors, but is quickly down-regulated in postmitotic oligodendrocytes (A007(+)/galacto-cerebroside(+)) along with A2B5 and vimentin. However, other glial cells including type 2 astrocytes and some amoeboid microglia alsoshare nestin expression. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 08:48:09