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Titolo:
Effect of the degree of fatty acid unsaturation of rat heart mitochondria on their rates of H2O2 production and lipid and protein oxidative damage
Autore:
Herrero, A; Portero-Otin, M; Bellmunt, MJ; Pamplona, R; Barja, G;
Indirizzi:
Univ Complutense, Fac Biol, Dept Anim Biol Anim Physiol 2, E-28040 Madrid,Spain Univ Complutense Madrid Spain E-28040 im Physiol 2, E-28040 Madrid,Spain Lleida Univ, Fac Med, Dept Basic Med Sci, Metab Physiopathol Res Grp, Lleida 25198, Spain Lleida Univ Lleida Spain 25198 Physiopathol Res Grp, Lleida 25198, Spain
Titolo Testata:
MECHANISMS OF AGEING AND DEVELOPMENT
fascicolo: 4, volume: 122, anno: 2001,
pagine: 427 - 443
SICI:
0047-6374(20010415)122:4<427:EOTDOF>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
FREE-RADICAL PRODUCTION; MAXIMUM LIFE-SPAN; LIVER-MITOCHONDRIA; TISSUE PROTEINS; PEROXIDATION; PIGEON; GENERATION; LONGEVITY; MAMMALS; STRESS;
Keywords:
polyunsaturated fatty acids; free radical generation; protein carbonyls; ageing; malondialdehyde; lipid peroxidation; hydrogen peroxide; carboxymethyllysine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Barja, G Univ Complutense, Fac Biol, Dept Anim Biol Anim Physiol 2, E-28040 Madrid,Spain Univ Complutense Madrid Spain E-28040 l 2, E-28040 Madrid,Spain
Citazione:
A. Herrero et al., "Effect of the degree of fatty acid unsaturation of rat heart mitochondria on their rates of H2O2 production and lipid and protein oxidative damage", MECH AGE D, 122(4), 2001, pp. 427-443

Abstract

Previous comparative studies have shown that long-lived animals have lowerfatty acid double bond content in their mitochondrial membranes than short-lived ones. In order to ascertain whether this trait protects mitochondriaby decreasing lipid and protein oxidation and oxygen radical generation, the double bond content of rat heart mitochondrial membranes was manipulatedby chronic feeding with semi-purified AIN-93G diets rich in highly unsaturated (UNSAT) or saturated (SAT) oils. UNSAT rat heart mitochondria had significantly higher double bond content and lipid peroxidation than SAT mitochondria. They also showed increased levels of the markers of protein oxidative damage malondialdehyde-lysine, protein carbonyls, and N-e-(carboxymethyl)lysine adducts. Basal rates of mitochondrial oxygen radical generation were not modified by the degree of fatty acid unsaturation, but the rates of H2O2 generation stimulated by antimycin A were higher in UNSAT than in SAT mitochondria. These results demonstrate that increasing the degree of fatty acid unsaturation of heart mitochondria increases oxidative damage to theirlipids and proteins, and can also increase their rates of mitochondrial oxygen radical generation in situations in which the degree of reduction of Complex III is higher than normal. These observations strengthen the notion that the relatively low double bond content of the membranes of long-lived animals could have evolved to protect them from oxidative damage. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 07:15:28