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Titolo:
Scanning near field optical microscopy of phase separated regions in a mixed interfacial protein (BSA)-surfactant (Tween 20) film
Autore:
Gunning, AP; Mackie, AR; Kirby, AR; Morris, VJ;
Indirizzi:
Food Res Inst, Norwich NR4 7UA, Norfolk, England Food Res Inst Norwich Norfolk England NR4 7UA h NR4 7UA, Norfolk, England
Titolo Testata:
LANGMUIR
fascicolo: 6, volume: 17, anno: 2001,
pagine: 2013 - 2018
SICI:
0743-7463(20010320)17:6<2013:SNFOMO>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
ATOMIC-FORCE MICROSCOPY; OROGENIC DISPLACEMENT; AIR/WATER INTERFACE; MONOLAYERS; ARTIFACTS; SCALE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Gunning, AP Food Res Inst, Norwich Res Pk, Norwich NR4 7UA, Norfolk, England Food Res Inst Norwich Res Pk Norwich Norfolk England NR4 7UA d
Citazione:
A.P. Gunning et al., "Scanning near field optical microscopy of phase separated regions in a mixed interfacial protein (BSA)-surfactant (Tween 20) film", LANGMUIR, 17(6), 2001, pp. 2013-2018

Abstract

Scanning near field optical microscopy (SNOM) has been used to visualize phase-separated regions in a monolayer protein film. The film was created atan air-water interface during displacement of the protein bovine serum albumin (BSA) by the nonionic surfactant Tween 20. Contrast was generated in the SNOM images By fluorescent labeling of the protein. The validity of the SNOM methodology has been confirmed by a qualitative and quantitative comparison of the displacement process as visualized by SNOM and atomic force microscopy (AFM) on fluorescently labeled BSA displaced by Tween 20 and AFM data on native BSA displaced by Tween 20. The data suggest that SNOM studiesof labeled proteins can be used to investigate the behavior of particular protein species in complex multicomponent interfacial films.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 11:51:39