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Titolo:
Axonal transport of TNF-alpha in painful neuropathy: distribution of ligand tracer and TNF receptors
Autore:
Shubayev, VI; Myers, RR;
Indirizzi:
Univ Calif San Diego, Dept Anesthesiol, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 hesiol, La Jolla, CA 92093 USA VA Healthcare Ctr, San Diego, CA USA VA Healthcare Ctr San Diego CA USAVA Healthcare Ctr, San Diego, CA USA
Titolo Testata:
JOURNAL OF NEUROIMMUNOLOGY
fascicolo: 1-2, volume: 114, anno: 2001,
pagine: 48 - 56
SICI:
0165-5728(20010301)114:1-2<48:ATOTIP>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; PERIPHERAL-NERVE INJURY; NEURONAL CELL-DEATH; ANTEROGRADE TRANSPORT; RETROGRADE TRANSPORT; NEUROTROPHIC FACTOR; SENSORY NEURONS; EXPRESSION; AXOTOMY; APOPTOSIS;
Keywords:
biotinylated TNF-alpha; TNF receptor; retrograde axonal transport; peripheral nerve injury; pain;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Shubayev, VI Univ Calif San Diego, Dept Anesthesiol, 9500 Gilman Dr, La Jolla, CA 92093USA Univ Calif San Diego 9500 Gilman Dr La Jolla CA USA 92093 USA
Citazione:
V.I. Shubayev e R.R. Myers, "Axonal transport of TNF-alpha in painful neuropathy: distribution of ligand tracer and TNF receptors", J NEUROIMM, 114(1-2), 2001, pp. 48-56

Abstract

Tumor necrosis factor-alpha (TNF-alpha) is a key player in peripheral nerve injury. In the inflammatory chronic constriction injury (CCI) model of sciatic neuropathy, upregulation of TNF-alpha mRNA and protein at the site ofnerve injury has been associated with pain. We now report the distributionof endogenous TNF-alpha protein and its receptors along normal and CCI-injured sciatic nerves, and within the corresponding lumbar dorsal root gan,alia (DRG). Using Western blotting, TNF-alpha was found to be distinctly increased at the injury site, as well as in the axons just distal to the corresponding DRG. The TNF-alpha. signal between the injury site and DRG (midaxonal) was induced between 2 and 5 days post-CCI, suggesting activation of TNF-alpha axonal transport. Endogenous TNF-a was localized in small-diameter, presumably nociceptive, and large-diameter, presumably mechanoceptive, DRG sensory neurons in both normal and CCI animals. Intraneural microinjection of biotin-labeled TNF-alpha showed specific axonal uptake at the injection site, as detected by avidin-biotinperoxidase histochemistry, and confirmed by co-localization with neurobiotin tracer. In control animals, fast retrograde transport of biotinylated TNF-alpha to both L4 and L5 DRG neurons was apparent 6 h following injection. TNF receptors TNFRI and TNFRII co-localized with biotinylated TNF-alpha tracer along the nerve trunk, suggesting that TNF-alpha, transport may be receptor-mediated. In animals with CCI neuropathy, uptake of biotinylated TNF-alpha by neuronal soma was inhibited. Instead, there was signal accumulation in the axons immediately distal to the DRG, and TNFRI and RII were increased at this same anatomic location. These findings highlight a dynamic process of TNF-alpha. protein and receptor regulation throughout the peripheral neural axis that bears on both the normalfunction of DRG neurons and the pathogenesis of painful neuropathies. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 22:54:53