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Titolo:
Vitreal dihydroxyphenylacetic acid (DOPAC) as an index of retinal dopaminerelease
Autore:
Megaw, P; Morgan, I; Boelen, M;
Indirizzi:
La Trobe Univ, Div Biol Sci, Bendigo, Vic 3552, Australia La Trobe Univ Bendigo Vic Australia 3552 ci, Bendigo, Vic 3552, Australia Australian Natl Univ, Res Sch Biol Sci, Visual Sci Grp, Canberra, ACT 2601, Australia Australian Natl Univ Canberra ACT Australia 2601 rra, ACT 2601, Australia Australian Natl Univ, Ctr Visual Sci, Canberra, ACT 2601, Australia Australian Natl Univ Canberra ACT Australia 2601 rra, ACT 2601, Australia
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 6, volume: 76, anno: 2001,
pagine: 1636 - 1644
SICI:
0022-3042(200103)76:6<1636:VDA(AA>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOGENOUS DOPAMINE; CHICK RETINA; FISH RETINA; RAT-BRAIN; LIGHT; METABOLISM; 3-METHOXYTYRAMINE; LOCALIZATION; STIMULATION; TURNOVER;
Keywords:
alpha-methyl-para-tyrosine; amacrine cells; chicken; dopamine synthesis; light adaptation; tyrosine hydroxylase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Boelen, M La Trobe Univ, Div Biol Sci, POB 199, Bendigo, Vic 3552, Australia La Trobe Univ POB 199 Bendigo Vic Australia 3552 3552, Australia
Citazione:
P. Megaw et al., "Vitreal dihydroxyphenylacetic acid (DOPAC) as an index of retinal dopaminerelease", J NEUROCHEM, 76(6), 2001, pp. 1636-1644

Abstract

Dopamine is generally accepted as a major neurotransmitter associated withlight-adaptive processes in the retina. However, little is known about itsprecise release pattern in vivo, largely due to the lack of an unambiguousmethod for the determination of dopamine release. We have found that vitreal levels of dihydroxyphenylacetic acid (DOPAC) reflect the rate of dopamine release in chickens. Blocking re-uptake with nomifensine significantly lowered vitreal DOPAC and retinal dopamine, confirming the retinal origin andreliance of vitreal DOPAC on intact re-uptake mechanisms. Further, inhibition of monoamine oxidase with pargyline reduced vitreal as well as retinal DOPAC levels, confirming that the DOPAC detected is generated by monoamine oxidase. Finally, we found that DOPAC diffused freely into and out of isolated vitreous bodies and we found the vitreous to be metabolically inert with respect to DOPAC, supporting the idea that vitreal levels of DOPAC are consequential to the retinal metabolism of dopamine. Exposure to light, whichis known to increase retinal dopamine release, readily increased vitreal DOPAC levels. The accumulation of DOPAC in the vitreous over 6 h light fitted a mathematical model of DOPAC accumulation based on zero-order influx (proportional to dopamine release rates) and diffusion driven, first-order efflux.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 01:54:42