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Titolo:
Homotypic cluster formation of dendritic cells, a close correlate of theirstate of maturation. Defects in the biobreeding diabetes-prone rat
Autore:
Delemarre, FGA; Hoogeveen, PG; de Haan-Meulman, M; Simons, PJ; Drexhage, HA;
Indirizzi:
Erasmus Univ, Fac Med, Dept Immunol, Lab EE838, NL-3000 DR Rotterdam, Netherlands Erasmus Univ Rotterdam Netherlands NL-3000 DR DR Rotterdam, Netherlands
Titolo Testata:
JOURNAL OF LEUKOCYTE BIOLOGY
fascicolo: 3, volume: 69, anno: 2001,
pagine: 373 - 380
SICI:
0741-5400(200103)69:3<373:HCFODC>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
CD34(+) HEMATOPOIETIC PROGENITORS; MIXED LYMPHOCYTE-REACTION; ANTIGEN-PRESENTING CELLS; LANGERHANS CELLS; AIRWAY EPITHELIUM; DISEASE; GROWTH; ALPHA; CD43; DIFFERENTIATION;
Keywords:
cellular aggregates; BB-DP rat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Drexhage, HA Erasmus Univ, Fac Med, Dept Immunol, Lab EE838, POB 1738, NL-3000 DR Rotterdam, Netherlands Erasmus Univ POB 1738 Rotterdam NetherlandsNL-3000 DR lands
Citazione:
F.G.A. Delemarre et al., "Homotypic cluster formation of dendritic cells, a close correlate of theirstate of maturation. Defects in the biobreeding diabetes-prone rat", J LEUK BIOL, 69(3), 2001, pp. 373-380

Abstract

Aggregation of dendritic cells (DCs) in homotypic clusters has been described in vivo in lymph and shin, and here we report studies on homotypic clustering of rat splenic (s) DCs in vitro. Wister rat sDCs readily formed homotypic clusters in culture, which increased in number and size over time (with a peak at t = 3 h). Keeping the cells at higher densities or treatment with anti-CD43 induced more and larger homotypic clusters, After such enhanced clustering the DCs had increased their T cell stimulating capabilities in syngeneic mixed lymphocyte reaction, and had a higher expression of CD80 and CD86 (signs of maturation). Ag transfer from bovine serum albumin-fluorescein isothiocyanate-pulsed to unpulsed DCs was observed during clustering. Here we also show that sDCs of the biobreeding diabetes-prone (BB-DP) rat, a model of autoimmune diabetes/thyroiditis, formed fewer and smaller clusters than Wistar sDCs, and that DC-DC clustering resulted in only a modest maturation of the cells (as determined in syn MLR and by phenotyping). Anti-CD43 completely restored the clustering defect BB-DP DCs in vitro, yet T cell-stimulating capability was only restored to a limited extent. Ag transfer in BB-DP DC clusters was similar.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 18:32:44