Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Acute rejection and cardiac graft vasculopathy in the absence of donor-derived ICAM-1 or P-selectin
Autore:
Raisky, O; Morrison, KJM; Obadia, JF; McGregor, J; Yacoub, MH; Rose, ML;
Indirizzi:
Natl Heart & Lung Inst, Harefield Hosp, Harefield UB9 6JH, Middx, England Natl Heart & Lung Inst Harefield Middx England UB9 6JH JH, Middx, England Imperial Coll, Sch Med, Royal Brompton & Harefield NHS Trust, Harefield UB9 6JH, Middx, England Imperial Coll Harefield Middx England UB9 6JH eld UB9 6JH, Middx, England Hop Cardiovasc & Pneumol, Lyon, France Hop Cardiovasc & Pneumol Lyon France Cardiovasc & Pneumol, Lyon, France
Titolo Testata:
JOURNAL OF HEART AND LUNG TRANSPLANTATION
fascicolo: 3, volume: 20, anno: 2001,
pagine: 340 - 349
SICI:
1053-2498(200103)20:3<340:ARACGV>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERCELLULAR-ADHESION MOLECULE-1; TRANSPLANT ARTERIOSCLEROSIS; ATHEROSCLEROTIC PLAQUES; ALLOGRAFT SURVIVAL; T-HELPER-2 CELLS; KNOCKOUT MICE; EXPRESSION; RECIPIENTS; DEFICIENT; LFA-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Rose, ML Natl Heart & Lung Inst, Harefield Hosp, Harefield UB9 6JH, Middx,England Natl Heart & Lung Inst Harefield Middx England UB9 6JH , England
Citazione:
O. Raisky et al., "Acute rejection and cardiac graft vasculopathy in the absence of donor-derived ICAM-1 or P-selectin", J HEART LUN, 20(3), 2001, pp. 340-349

Abstract

Background: ICAM-1 and P-selectin are molecules that facilitate adhesion of circulating leukocytes to vessel walls. We have investigated the role of donor-derived ICAM-1 and P-selectin in acute and chronic cardiac allograft rejection. Methods: C57BL/6J (H-2(b)) mice were used as donors for heterotopic heart transplantation into CBA/Ca (H-2(k)) recipients. The donors were wild-type or homozygous for gene mutations of ICAM-1 or P-selectin. We measured acuterejection in non-immunosuppressed recipients by daily palpation and sacrificed mice at Days 2, 4, and 6 for immunohistochemical analysis. For chronicrejection, recipients received monoclonal antibody against CD4+ T cells. We removed hearts at Days 60 to 62 for histologic assessment of vasculopathyusing quantitative morphometry to measure intimal thickening. Results: Time (days) to rejection was 7.1 +/- 0.57 for wild-type (n = 10),7.0 +/- 0.71 for ICAM-1 -/- (not significantly different, n = 7) and 6.1 +/- 0.33 (p = 0.001) for P-selectin -/- donors. ICAM-1 deficiency was associated with delayed infiltrate at Day 4 compared with wild-type. In the modelof chronic rejection, elastin-positive vessels showed a mean occlusion of 34% +/- 3% in transplanted wild-type hearts; vessels were divided into those showing 0% to 20%, 20% to 50%, and 50% to 100% occlusion. We observed no difference in the number of affected vessels or the amount of vascular thickening in donors lacking ICAM-1 or P-selectin compared with wild-type controls. Conclusions: The absence of ICAM-1 or P-selectin in donor tissues neither lengthens the time of allograft survival nor inhibits the vascular lesions associated with chronic rejection. Indeed, the absence of P-selectin may exacerbate alloimmune injury.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 02:35:54