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Titolo:
Structural changes of human serum albumin immobilized on chromatographic supports: a high-performance liquid chromatography and Fourier-transform infrared spectroscopy study
Autore:
Millot, MC; Servagent-Noinville, S; Taleb, NL; Baron, MH; Revault, M; Sebille, B;
Indirizzi:
Univ Paris 12, Lab Rech Polymeres, CNRS, UMR 7581, F-94320 Thiais, France Univ Paris 12 Thiais France F-94320 RS, UMR 7581, F-94320 Thiais, France Univ Paris 06, Lab Dynam Interact & React, CNRS, UMR 7075, F-94320 Thiais,France Univ Paris 06 Thiais France F-94320 NRS, UMR 7075, F-94320 Thiais,France
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 753, anno: 2001,
pagine: 101 - 113
SICI:
1387-2273(20010325)753:1<101:SCOHSA>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
REVERSED-PHASE SUPPORT; SILICA; RESOLUTION; ADSORPTION; SEPARATION; RETENTION; PROTEINS; COLUMNS; DRUGS;
Keywords:
human serum albumin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Millot, MC Univ Paris 12, Lab Rech Polymeres, CNRS, UMR 7581, 2 Rue Henri Dunant, F-94320 Thiais, France Univ Paris 12 2 Rue Henri Dunant Thiais France F-94320 France
Citazione:
M.C. Millot et al., "Structural changes of human serum albumin immobilized on chromatographic supports: a high-performance liquid chromatography and Fourier-transform infrared spectroscopy study", J CHROMAT B, 753(1), 2001, pp. 101-113

Abstract

Chiral stationary phases obtained by immobilization of HSA on [C8] and [C18] reversed-phases and on poly(1-vinylimidazole)-coated silica were tested to resolve DL-tryptophan, N-benzoyl-DL-phenylalanine, RS-oxazepam and RS-warfarin racemic mixtures. Parameters of enantioselectivity measured in HPLC are correlated to structural and solvation states for adsorbed HSA, evaluated by FTIR spectroscopy. HSA immobilized on [PVI]-anion-exchangers is highly selective. HSA molecules are not self-associated, only unfolded for a small hydrophobic helix. The HSA-coated reversed-phases have a lower selectivity. Unfolding is larger but the indole-benzodiazepine chiral site is preserved and remains accessible. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 19:01:18