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Titolo:
Chromosomal control of albumins and globulins in wheat grain assessed using different fractionation procedures
Autore:
Singh, J; Skerritt, JH;
Indirizzi:
Qual Wheat Coopeat Res Ctr, Canberra, ACT 2601, Australia Qual Wheat Coopeat Res Ctr Canberra ACT Australia 2601 CT 2601, Australia CSIRO Plant Ind, Canberra, ACT 2601, Australia CSIRO Plant Ind Canberra ACT Australia 2601 Canberra, ACT 2601, Australia
Titolo Testata:
JOURNAL OF CEREAL SCIENCE
fascicolo: 2, volume: 33, anno: 2001,
pagine: 163 - 181
SICI:
0733-5210(200103)33:2<163:CCOAAG>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOLECULAR-WEIGHT ALBUMINS; TRITICUM-AESTIVUM L; ALPHA-AMYLASE; GROUP-1 CHROMOSOMES; SOLUBLE PROTEINS; BETA-AMYLASE; BREAD WHEAT; DURUM-WHEAT; GENES; ENDOSPERM;
Keywords:
wheat; proteins; albumins; globulins;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Skerritt, JH Australian Ctr Int Agr Res, GPO Box 1571, Canberra, ACT 2601,Australia Australian Ctr Int Agr Res GPO Box 1571 Canberra ACT Australia 2601
Citazione:
J. Singh e J.H. Skerritt, "Chromosomal control of albumins and globulins in wheat grain assessed using different fractionation procedures", J CEREAL SC, 33(2), 2001, pp. 163-181

Abstract

A systematic study of the water-soluble (albumin) and salt-soluble (globulin) proteins of wheat flour, by analysis of null genetic lines using different methods of protein separation, has established the location of several of their genes on individual chromosomes. SDS-PAGE analysis of water-soluble proteins indicated the chromosomal location of polypeptides of M-chi, 64 000, M-chi 62 000 and M-chi, 16 000 on 4DL, 4BS and 3DS respectively. Similarly, two globulin polypeptides of M-chi 38 000 and M-chi, 39 000 were assigned to chromosomes 2A, 2B, 2D, 3BS, 3DS, 4DS, 5DL, 6DS, 7BS or 7DL using isoelectric focusing. In addition. several non-gluten proteins have been mapped on chromosome 1D, 3AL, 3BS, 3DS, 4AL, 4BS, 4DS, and 5DL by reversed-phase HPLC (RP-HPLC). The proteins separated hy RP-HPLC were also correlated with proteins resolved with other methods. The polypeptides assigned to different chromosomes using SDS-PAGE and isoelectric focusing were also analysed by 2-D electrophoresis to confirm the chromosomal location of genes controlling expression of particular polypeptides. Some other proteins that werenot resolved by either method alone, were able to be mapped on chromosomesusing 2-D electrophoresis. The chromosome-specific proteins are now being used to develop chromosome-specific markers, which may be valuable to discover spontaneous deletions as well as natural translocations from alien species. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 07:28:00