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Titolo:
Growth and form of secretory granules involves stepwise assembly but not differential sorting of a family of secretory proteins in Paramecium
Autore:
Vayssie, L; de Loubresse, NG; Sperling, L;
Indirizzi:
CNRS, Ctr Mol Genet, F-91198 Gif Sur Yvette, France CNRS Gif Sur Yvette France F-91198 Genet, F-91198 Gif Sur Yvette, France
Titolo Testata:
JOURNAL OF CELL SCIENCE
fascicolo: 5, volume: 114, anno: 2001,
pagine: 875 - 886
SICI:
0021-9533(200103)114:5<875:GAFOSG>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANS-GOLGI NETWORK; TETRAHYMENA-THERMOPHILA; TRICHOCYST FORMATION; LOCALIZATION; BIOGENESIS; INSULIN; MATRIX; RAT; POLYPEPTIDES; MATURATION;
Keywords:
dense core vesicle; multigene family; gene silencing; proteolytic processing; membrane traffic; trichocyst;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Sperling, L CNRS, Ctr Mol Genet, F-91198 Gif Sur Yvette, France CNRS Gif Sur Yvette France F-91198 98 Gif Sur Yvette, France
Citazione:
L. Vayssie et al., "Growth and form of secretory granules involves stepwise assembly but not differential sorting of a family of secretory proteins in Paramecium", J CELL SCI, 114(5), 2001, pp. 875-886

Abstract

Paramecium trichocysts are voluminous secretory vesicles consisting of a spindle-shaped body surmounted by a tip that serves to anchor them at exocytotic sites in the plasma membrane. This constrained shape is conferred by the proteins stored in the vesicles, which form an insoluble three-dimensional crystalline array. The constituent polypeptides (Trichocyst Matrix Proteins, TMPs), which assemble during trichocyst biogenesis, are produced by proteolytic processing of soluble proproteins encoded by a large multigene family. In order to investigate the functional significance of the TMP multigene family, which assures the synthesis of a mixture of related polypeptides, we have designed synthetic genes for heterologous expression of three different mature polypeptides, which were used to obtain sequence-specific rabbit antisera, We used these antisera to carry out immunolocalization experiments with wild-type trichocysts at different stages of development and found that the trichocyst matrix consists of two concentric layers containingdifferent TMPs, and that the assembly of each layer corresponds to a distinct phase of trichocyst growth. Examination of mutant trichocysts created by targeted gene silencing of different TMP genes showed that the layer containing the products of the silenced genes is specifically affected, as are all subsequently assembled parts of the structure, consistent with an ordered assembly pathway. This stepwise assembly is not controlled by differential sorting of the TMPs, as single and double label experiments provided evidence that the different TMPs are delivered together to post-Golgi vesiclesand developing trichocysts. We present a model for trichocyst biogenesis in which TMP assembly is controlled by protein processing.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 22:14:26