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Titolo:
Increased vitamin D receptor level enhances 1,25-dihydroxyvitamin D-3-mediated gene expression and calcium transport in Caco-2 cells
Autore:
Shao, A; Wood, RJ; Fleet, JC;
Indirizzi:
Purdue Univ, Dept Foods & Nutr, W Lafayette, IN 47907 USA Purdue Univ W Lafayette IN USA 47907 ds & Nutr, W Lafayette, IN 47907 USA Tufts Univ, USDA, Human Nutr Res Ctr Aging, Mineral Bioavailabil Lab, Boston, MA 02111 USA Tufts Univ Boston MA USA 02111 ral Bioavailabil Lab, Boston, MA 02111 USA
Titolo Testata:
JOURNAL OF BONE AND MINERAL RESEARCH
fascicolo: 4, volume: 16, anno: 2001,
pagine: 615 - 624
SICI:
0884-0431(200104)16:4<615:IVDRLE>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
OSTEOBLAST-LIKE CELLS; MESSENGER-RIBONUCLEIC-ACID; PARATHYROID-HORMONE; BINDING PROTEIN; RNA EXPRESSION; LINE CACO-2; WISTAR RATS; AGE; ABSORPTION; D-3;
Keywords:
vitamin D receptor; 24-hydroxylase; calbindin D-9k; calcium transport; enterocyte; 1,25-dihydroxyvitamin D-3;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Shao, A Purdue Univ, Dept Foods & Nutr, 1264 Stone Hall,Room G1A, W Lafayette, IN 47907 USA Purdue Univ 1264 Stone Hall,Room G1A W Lafayette IN USA 47907 USA
Citazione:
A. Shao et al., "Increased vitamin D receptor level enhances 1,25-dihydroxyvitamin D-3-mediated gene expression and calcium transport in Caco-2 cells", J BONE MIN, 16(4), 2001, pp. 615-624

Abstract

Altered vitamin D receptor (VDR) level has been proposed to explain differences in intestinal responsiveness to 1,25-dihydroxyvitamin D-3 [1,25(OH)(2)D-3], We tested whether the enterocyte VDR level influences 1,25(OH)(2)D-3-mediated gene expression and transepithelial calcium (Ca) transport in thehuman intestinal cell line Caco-2, Cells were stably transfected with a human metallothionein (hMT) IIA promoter-human VDR (hVDR) complementary DNA (cDNA) transgene that overexpressed hVDR in response to heavy metals. In MTVDR clones, induction of 25-hyroxyvitamin D-3-24-hydroxylase (24-OHase) messenger RNA (mRNA) expression by 1,25(OH)(2)D-3 (10(-9) M, 4 h) was correlated to metal-induced changes in nuclear VDR level (r(2) = 0.99), In MTVDR clones, basal VDR level was 2-fold greater and 1,25(OH)(2)D-3-mediated Ca transport (10(-7) M, 24 h) was 43% higher than in parental Caco-2 cells. Treatment of MTVDR clones with Cd (1 muM, 28 h) increased VDR level by 68%, significantly enhanced 1,25(OH)(2)D-3-mediated Ca transport by 24%, and increased accumulation of calbindin D-9K mRNA by 76% relative to 1,25(OH)(2)D-3 alone. These observations support the hypothesis that the enterocyte VDR levelis an important modulator of intestinal responsiveness to 1,25(OH)(2)D-3.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 06:34:05