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Titolo:
Pentanoic acid, a novel protein synthesis stimulant for Chinese hamster ovary (CHO) cells
Autore:
Liu, CH; Chu, IM; Hwang, SM;
Indirizzi:
Natl Tsing Hua Univ, Dept Chem Engn, Hsinchu 300, Taiwan Natl Tsing Hua Univ Hsinchu Taiwan 300 pt Chem Engn, Hsinchu 300, Taiwan Food Ind Res & Dev Inst, Hsinchu 300, Taiwan Food Ind Res & Dev Inst Hsinchu Taiwan 300 Dev Inst, Hsinchu 300, Taiwan
Titolo Testata:
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
fascicolo: 1, volume: 91, anno: 2001,
pagine: 71 - 75
SICI:
1389-1723(200101)91:1<71:PAANPS>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
SODIUM-BUTYRATE; ANTIBODY-PRODUCTION; EXPRESSION;
Keywords:
pentanoic acid; butyrate; protein synthesis stimulant; CHO cells; apoptosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Chu, IM Natl Tsing Hua Univ, Dept Chem Engn, Hsinchu 300, Taiwan Natl Tsing Hua Univ Hsinchu Taiwan 300 Engn, Hsinchu 300, Taiwan
Citazione:
C.H. Liu et al., "Pentanoic acid, a novel protein synthesis stimulant for Chinese hamster ovary (CHO) cells", J BIOSCI BI, 91(1), 2001, pp. 71-75

Abstract

Nine carboxylic acids were tested to evaluate their effects on recombinantfusion protein production and cell growth of Chinese Hamster Ovary (CHO) cells. Pentanoic acid was demonstrated to have the highest enhancement effect on the protein biosynthesis of CHO cells among the acids tested. Pentanoic acid also had less growth suppression effects compared with butyrate. Theoptimal induction time and concentration of pentanoic acid for a 120-h batch culture were 72 h and 1 mM, respectively. Apoptosis (programmed cell death) was observed in the serum-free batch culture of CHO cells using a cell death detection ELISA kit. The addition of butyrate accelerated the rate ofapoptosis of CHO cells whereas the addition of pentanoate did not. These results confirmed that pentanoic acid was a better stimulant for protein biosynthesis in animal cell culture than butyrate.

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Documento generato il 02/04/20 alle ore 12:42:22