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Titolo:
Dosage effect of minor arginyl- and isoleucyl-tRNAs on protein synthesis in an Escherichia coli in vitro coupled transcription/translation system
Autore:
Jiang, XP; Nakano, H; Kigawa, T; Yabuki, T; Yokoyama, S; Clark, DS; Yamane, T;
Indirizzi:
Nagoya Univ, Grad Sch Biol & Agr Sci, Lab Mol Biotechnol, Chikusa Ku, Nagoya, Aichi 4648601, Japan Nagoya Univ Nagoya Aichi Japan 4648601 a Ku, Nagoya, Aichi 4648601, Japan RIKEN, Inst Phys & Chem Res, Genom Sci Res Ctr, Wako, Saitama, Japan RIKEN Wako Saitama Japan em Res, Genom Sci Res Ctr, Wako, Saitama, Japan RIKEN, Cellular Signaling Lab, Wako, Saitama, Japan RIKEN Wako Saitama Japan N, Cellular Signaling Lab, Wako, Saitama, Japan Univ Tokyo, Grad Sch Sci, Dept Biochem, Tokyo, Japan Univ Tokyo Tokyo Japan Tokyo, Grad Sch Sci, Dept Biochem, Tokyo, Japan Univ Calif Berkeley, Dept Chem Engn, Berkeley, CA 94720 USA Univ Calif Berkeley Berkeley CA USA 94720 em Engn, Berkeley, CA 94720 USA
Titolo Testata:
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
fascicolo: 1, volume: 91, anno: 2001,
pagine: 53 - 57
SICI:
1389-1723(200101)91:1<53:DEOMAA>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSFER-RNA; DISULFIDE BRIDGE; HIGHLY EFFICIENT; CODONS; GENES; AGA;
Keywords:
minor tRNA; cell-free protein synthesis; rare codon;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Nakano, H Nagoya Univ, Grad Sch Biol & Agr Sci, Lab Mol Biotechnol, Chikusa Ku, FuroCho, Nagoya, Aichi 4648601, Japan Nagoya Univ Furo Cho Nagoya Aichi Japan 4648601 i 4648601, Japan
Citazione:
X.P. Jiang et al., "Dosage effect of minor arginyl- and isoleucyl-tRNAs on protein synthesis in an Escherichia coli in vitro coupled transcription/translation system", J BIOSCI BI, 91(1), 2001, pp. 53-57

Abstract

In Escherichia coli mRNA, the arginine codons AGA/AGG and the isoleucine codon AUA are rarely used with frequencies of about 0.35% and 0.41%, respectively. Six genes with a different number of these codons were expressed in an E. coli in vitro coupled transcription/translation system, which contained either tRNA prepared from E. coli cells carrying a plasmid with argU andileX genes encoding rare tRNAs (tRNA(arg) AGA/AGG and tRNA(AUA)(ile)), designated codon-plus tRNA, or normal tRNA from cells lacking the plasmid. Genes having a low number of the rare codons, such as genes encoding chloramphenicol acetyltransferase and anti-gp120 single-chain Fv (artificially constructed to remove rare codons), were expressed at similar levels using with both tRNA preparations. On the other hand, the use of codon-plus tRNA increased the expression levels of genes having a relatively large number of therare codons, including genes encoding archaeal proteins, green fluorescentprotein of jelly fish origin, and a single-chain Fv of mouse origin, by about 20% higher than that using normal tRNA.

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Documento generato il 23/09/20 alle ore 13:16:13