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Titolo:
A new method to measure nitrate/nitrite with a NO-sensitive electrode
Autore:
Berkels, R; Purol-Schnabel, S; Roesen, R;
Indirizzi:
Univ Cologne, Inst Pharmakol, D-50931 Cologne, Germany Univ Cologne Cologne Germany D-50931 Pharmakol, D-50931 Cologne, Germany
Titolo Testata:
JOURNAL OF APPLIED PHYSIOLOGY
fascicolo: 1, volume: 90, anno: 2001,
pagine: 317 - 320
SICI:
8750-7587(200101)90:1<317:ANMTMN>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE RELEASE; ENDOTHELIAL-CELLS; RELAXING FACTOR; EDRF; VEIN;
Keywords:
nitric oxide; endothelium; nitrite;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Berkels, R Univ Cologne, Inst Pharmakol, Gleueler Str 24, D-50931 Cologne,Germany Univ Cologne Gleueler Str 24 Cologne Germany D-50931 , Germany
Citazione:
R. Berkels et al., "A new method to measure nitrate/nitrite with a NO-sensitive electrode", J APP PHYSL, 90(1), 2001, pp. 317-320

Abstract

There are different methods to measure the unstable molecule nitric oxide (NO). We will describe a new sensitive method to measure NO by reconversionof nitrate/nitrite to NO, which will be determined with an amperometric Clark-type electrode. Nitrate and nitrite are the degradation products of NO. First, nitrate is enzymatically converted to nitrite with the use of the nitrate reductase. Second, nitrite is reduced to equimolar NO concentrationsby an acidic iodide solution. The detection limit of the electrode in an aqueous solution was 2 nmol/l NO (meaning the threshold was depending on thevolume added: 500 mul of a 0.2 mu mol/l nitrite solution added to a 10-ml bath). This method provides the ability to assess basal and agonist-stimulated NO releases of different biological models. We measured basal and carbachol-stimulated NO release of native endothelial cells from porcine coronary arteries and porcine aortic endothelial cell cultures. Moreover, it was possible to measure the nitrate! nitrite concentration in the coronary effluent of a guinea pig heart. In conclusion, we present a valid, highly sensitive new method of measuring nitrite/NO in biological systems with a commercially available electrode.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 09:21:46