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Titolo:
Nucleus raphe obscurus modulates hypoglossal output of neonatal rat in vitro transverse brain stem slices
Autore:
Peever, JH; Necakov, A; Duffin, J;
Indirizzi:
Univ Toronto, Dept Physiol, Resp Neurosci Lab, Toronto, ON M5S 1A8, CanadaUniv Toronto Toronto ON Canada M5S 1A8 i Lab, Toronto, ON M5S 1A8, Canada Univ Toronto, Dept Anaesthesia, Resp Neurosci Lab, Toronto, ON M5S 1A8, Canada Univ Toronto Toronto ON Canada M5S 1A8 i Lab, Toronto, ON M5S 1A8, Canada
Titolo Testata:
JOURNAL OF APPLIED PHYSIOLOGY
fascicolo: 1, volume: 90, anno: 2001,
pagine: 269 - 279
SICI:
8750-7587(200101)90:1<269:NROMHO>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
VENTRAL RESPIRATORY GROUP; SPINAL-CORD PREPARATION; PRE-BOTZINGER COMPLEX; MEDULLARY RAPHE; SUBSTANCE-P; IN-VITRO; CENTRAL CHEMORECEPTION; RHYTHM GENERATION; NEWBORN RATS; NEURONS;
Keywords:
respiratory neurons; brain stem; pH/carbon dioxide;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Duffin, J Univ Toronto, Dept Physiol, Resp Neurosci Lab, 1 Kings Coll Circle, Toronto, ON M5S 1A8, Canada Univ Toronto 1 Kings Coll Circle Toronto ONCanada M5S 1A8 anada
Citazione:
J.H. Peever et al., "Nucleus raphe obscurus modulates hypoglossal output of neonatal rat in vitro transverse brain stem slices", J APP PHYSL, 90(1), 2001, pp. 269-279

Abstract

Nucleus raphe obscurus (NRo) modulates hypoglossal (WI) nerve motor outputin the in vitro transverse brain stem slice of neonatal rats (1-5 days old); chemical ablation of NRo and its focal CO2 acidification modulated the bursting rhythm of XII nerves. We microinjected a 4.5 mM solution of kainic acid into the NRo to disrupt cellular activity and observed that XII nerve activity was temporarily abolished (n = 10). We also microinjected CO2-acidified (pH = 6.00 +/- 0.01) artificial cerebrospinal fluid (aCSF) into the NRo (n = 6), the pre-Botzinger complex (PBC) (n = 6), as well as a control region in the lateral tegmental field equidistant to NRo, PBC, and the XII motor nuclei (n = 12). CO2 acidification of the control region had no effecton XII motor output. CO2 acidification of the NRo significantly (P < 0.05)increased the burst discharge frequency of XII nerves by 77%; integrated burst amplitude and burst duration increased by 64% and 52%, respectively. CO2 acidification of the PBC significantly (P < 0.05) increased the burst discharge frequency of XII nerves by 65%, but neither integrated burst amplitude nor burst duration changed. These results demonstrate that chemical ablation of the NRo can abolish XII nerve bursting rhythm and that Stimulationof the NRo with CO2-acidified aCSF can excite XII nerve bursting activity. From these observations, we conclude that, in transverse brain stem slices, the NRo contains pH/CO2-sensitive cells that modulate XII motor output.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 12:09:45