Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Effects of SNP, ouabain, and amiloride on electrical potential profile of isolated sheep pleura
Autore:
Hatzoglou, CH; Gourgoulianis, KI; Molyvdas, PA;
Indirizzi:
Univ Thessaly, Sch Med, Dept Physiol, Larissa 41222, Greece Univ ThessalyLarissa Greece 41222 , Dept Physiol, Larissa 41222, Greece Gren Hos Larissa, Larissa, Greece Gren Hos Larissa Larissa GreeceGren Hos Larissa, Larissa, Greece
Titolo Testata:
JOURNAL OF APPLIED PHYSIOLOGY
fascicolo: 4, volume: 90, anno: 2001,
pagine: 1565 - 1569
SICI:
8750-7587(200104)90:4<1565:EOSOAA>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
PARIETAL PLEURA; VISCERAL PLEURA; TRANSPORT; LIQUID; NA+; PERMEABILITY; EPITHELIUM;
Keywords:
physiology; pleural permeability; Ussing system; transpleural potential; sodium nitroprusside;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Gourgoulianis, KI Univ Thessaly, Sch Med, Dept Physiol, 22 Papakyriazi, Larissa 41222, Greece Univ Thessaly 22 Papakyriazi Larissa Greece 41222 reece
Citazione:
C.H. Hatzoglou et al., "Effects of SNP, ouabain, and amiloride on electrical potential profile of isolated sheep pleura", J APP PHYSL, 90(4), 2001, pp. 1565-1569

Abstract

The fluid and solute transport properties of pleural tissue were studied by using specimens of intact visceral and parietal pleura from adult sheep lungs. The samples were transferred to the laboratory in a Krebs-Ringer solution at 4 degreesC within 1 h from the death of the animal. The pleura was then mounted as a planar sheet in a Ussing-type chamber. The results that are presented in this study are the means of six different experiments. The spontaneous potential difference and the inhibitory effects of sodium nitroprusside (SNP), ouabain, and amiloride on transepithelial electrical resistance (R-TE) were measured. The spontaneous potential difference across parietal pleura was 0.5 +/- 0.1 mV, whereas that across visceral pleura was 0.4+/- 0.1 mV. R-TE of both pleura was very low: 22.02 +/- 4.1 Omega .cm(2) for visceral pleura and 22.02 +/- 3.5 Omega .cm(2) for parietal pleura. There was an increase in the R-TE when SNP was added to the serosal bathing solution of parietal pleura and to the serosal or mucosal bathing solution in visceral pleura. The same was observed when ouabain was added to the mucosal surface of visceral pleura and to either the mucosal or serosal surface of parietal pleura. Furthermore, there was an increase in R-TE when amiloride was added to the serosal bathing solution of parietal pleura. Consequently, the sheep pleura appears to play a role in the fluid and solute transport between the pleural capillaries and the pleural space. There results suggest that there is a Na+ and K+ transport across both the visceral and parietal pleura.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/06/20 alle ore 22:27:43