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Titolo:
Mechanical compression influences intracellular Ca2+ signaling in chondrocytes seeded in agarose constructs
Autore:
Roberts, SR; Knight, MM; Lee, DA; Bader, DL;
Indirizzi:
Univ London Queen Mary & Westfield Coll, IRC Biomed Mat, London E1 4NS, England Univ London Queen Mary & Westfield Coll London England E1 4NS S, England Univ Coll London, Inst Orthopaed, IRC Biomed Mat, Stanmore HA7 4LP, Middx,England Univ Coll London Stanmore Middx England HA7 4LP re HA7 4LP, Middx,England Univ London Queen Mary & Westfield Coll, Dept Engn, Med Engn Div, London E1 4NS, England Univ London Queen Mary & Westfield Coll London England E1 4NS S, England
Titolo Testata:
JOURNAL OF APPLIED PHYSIOLOGY
fascicolo: 4, volume: 90, anno: 2001,
pagine: 1385 - 1391
SICI:
8750-7587(200104)90:4<1385:MCIICS>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
ISOLATED ARTICULAR CHONDROCYTES; ACTIVATED ION CHANNELS; FLUID-FLOW; CARTILAGE; CALCIUM; DEFORMATION; CELLS; PRESSURE; MATRIX; STRAIN;
Keywords:
mechanotransduction; microscopy; gadolinium; chondrocyte deformation; strain;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Roberts, SR Univ London Queen Mary & Westfield Coll, IRC Biomed Mat, Mile End Rd, London E1 4NS, England Univ London Queen Mary & Westfield Coll MileEnd Rd London England E1 4NS
Citazione:
S.R. Roberts et al., "Mechanical compression influences intracellular Ca2+ signaling in chondrocytes seeded in agarose constructs", J APP PHYSL, 90(4), 2001, pp. 1385-1391

Abstract

Ca2+ signaling forms part of a possible mechanotransduction pathway by which chondrocytes may alter their metabolism in response to mechanical loading. In this study, a well-charac terized model system utilizing bovine articular chondrocytes embedded in 4% agarose constructs was used to investigatethe effect of physiological mechanical compressive strain applied after 1 and 3 days in culture. The intracellular Ca2+ concentration was measured byuse of the ratiometric Ca indicator indo 1-AM and confocal microscopy. A positive Ca2+ response was defined as a percent increase in Ca2+ ratio abovea preset threshold. A significantly greater percentage of cells exhibited a positive Ca2+ response in strained constructs compared with unstrained controls at both time points. In strained constructs, treatment with either Ga3+ or EGTA significantly reduced the number of positive Ca2+ responders compared with untreated controls. These results represent an important step in understanding the physiological role of intracellular Ca2+ in chondrocytes under mechanical compression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 02:05:43