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Titolo:
Effect of unilateral denervation on maximum specific force in rat diaphragm muscle fibers
Autore:
Geiger, PC; Cody, MJ; Macken, RL; Bayrd, ME; Sieck, GC;
Indirizzi:
Mayo Clin & Mayo Fdn, Dept Anesthesiol, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn Rochester MN USA 55905 siol, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA MayoClin & Mayo Fdn Rochester MN USA 55905 phys, Rochester, MN 55905 USA
Titolo Testata:
JOURNAL OF APPLIED PHYSIOLOGY
fascicolo: 4, volume: 90, anno: 2001,
pagine: 1196 - 1204
SICI:
8750-7587(200104)90:4<1196:EOUDOM>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
MYOSIN HEAVY-CHAIN; RABBIT PSOAS FIBERS; SKELETAL-MUSCLE; CONTRACTILE PROPERTIES; HINDLIMB SUSPENSION; RESPIRATORY MUSCLES; ISOFORMS; ADAPTATIONS; HEMIDIAPHRAGM; ACTIVATION;
Keywords:
inactivity; skinned fibers; myosin heavy chain content; force per cross bridge;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Sieck, GC Mayo Clin & Mayo Fdn, Dept Anesthesiol, 200 1st St SW, Rochester, MN 55905USA Mayo Clin & Mayo Fdn 200 1st St SW Rochester MN USA 55905 905USA
Citazione:
P.C. Geiger et al., "Effect of unilateral denervation on maximum specific force in rat diaphragm muscle fibers", J APP PHYSL, 90(4), 2001, pp. 1196-1204

Abstract

We hypothesize that 1) the effect of denervation (DNV) is more pronounced in fibers expressing fast myosin heavy chain (MHC) isoforms and 2) the effect of DNV on maximum specific force reflects a reduction in MHC content perhalf sarcomere or the number of cross bridges in parallel. Studies were performed on single Triton X-100-permeabilized fibers activated at a pCa (-log Ca2+ concentration) of 4.0. MHC content per half sarcomere was determinedby densitometric analysis of SDS-PAGE gels and comparison to a standard curve of known MHC concentrations. After 2 of wk DNV, the maximum specific force of fibers expressing MHC2x was reduced by similar to 40% (MHC2x expression was absent), whereas the maximum specific force of fibers expressing MHC2A and MHCslow decreased by only similar to 20%. DNV also reduced the MHC content in fibers expressing MHC2x with no effect on fibers expressing MHC2A and MHCslow. When normalized for MHC content per half sarcomere, force generated by DNV fibers expressing MHC2x and MHC2A was decreased compared with control fibers. These results suggest the force per cross bridge is also affected by DNV.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 17:26:20