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Titolo:
Burkholderia cepacia genomovar VI, a new member of the Burkholderia cepacia complex isolated from cystic fibrosis patients
Autore:
Coenye, T; LiPuma, JJ; Henry, D; Hoste, B; Vandemeulebroecke, K; Gillis, M; Speert, DP; Vandamme, P;
Indirizzi:
State Univ Ghent, Microbiol Lab, B-9000 Ghent, Belgium State Univ Ghent Ghent Belgium B-9000 crobiol Lab, B-9000 Ghent, Belgium State Univ Ghent, BCCM LMG Bacteria Collect, B-9000 Ghent, Belgium State Univ Ghent Ghent Belgium B-9000 ria Collect, B-9000 Ghent, Belgium Univ Michigan, Dept Pediat & Communicable Dis, Sch Med, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 is, Sch Med, Ann Arbor, MI 48109 USA Univ British Columbia, Dept Pediat, Div Infect Dis & Immunol, Vancouver, BC V6T 1W5, Canada Univ British Columbia Vancouver BC Canada V6T 1W5 ver, BC V6T 1W5, Canada
Titolo Testata:
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
, volume: 51, anno: 2001,
parte:, 2
pagine: 271 - 279
SICI:
1466-5026(200103)51:<271:BCGVAN>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
SP-NOV; DEOXYRIBONUCLEIC-ACID; PSEUDOMONAS-CEPACIA; IDENTIFICATION; TRANSMISSION; GENUS;
Keywords:
Burkholderia cepacia complex; cystic fibrosis; taxonomy; genomovar;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Coenye, T State Univ Ghent, Microbiol Lab, KL Ledeganckstr 35, B-9000 Ghent, Belgium State Univ Ghent KL Ledeganckstr 35 Ghent Belgium B-9000 elgium
Citazione:
T. Coenye et al., "Burkholderia cepacia genomovar VI, a new member of the Burkholderia cepacia complex isolated from cystic fibrosis patients", INT J SY EV, 51, 2001, pp. 271-279

Abstract

A polyphasic taxonomic study was performed on 23 strains isolated from cystic fibrosis (CF) patients in the USA. These strains were tentatively identified as Burkholderia cepacia, Burkholderia vietnamiensis and Burkholderia or Ralstonia sp, using biochemical tests and 165 rDNA-based PCR assays. Visual comparison of protein profiles indicated that they belonged to a singlenew group ('group 13'). The polyphasic taxonomic data showed that 18 of these strains represent a new member of the B. cepacia complex, referred to in this report as B. cepacia genomovar VI, whereas the other five strains belonged to Burkholderia multivorans. By means of biochemical tests, B, cepacia genomovar VI strains can be separated from B. cepacia genomovars I and III, Burkholderia stabilis, B, vietnamiensis and Burkholderia gladioli, but not from B, multivorans. Separation of B, cepacia genomovar VI and B, multivorans is possible using AFLP (amplified fragment length polymorphism) fingerprinting and DNA-DNA hybridizations. Retrospective analysis of epidemiological and genotypic data suggests that strains of B, cepacia genomovar VI have been involved in chronic colonization of CF patients and have been spread from person to person.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/08/20 alle ore 00:13:00