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Titolo:
The invasive features of glial and non-central nervous system tumor cells are different on organotypic brain slices from newborn rats
Autore:
Murakami, M; Goto, S; Yoshikawa, M; Goto, T; Hamasaki, T; Rutka, JT; Kuratsu, JI; Ushio, Y;
Indirizzi:
Kumamoto Univ, Sch Med, Dept Neurosurg, Kumamoto 860, Japan Kumamoto UnivKumamoto Japan 860 ed, Dept Neurosurg, Kumamoto 860, Japan Univ Toronto, Hosp Sick Children, Brain Tumor Res Lab 1 1 2, Toronto, ON M5G 1X8, Canada Univ Toronto Toronto ON Canada M5G 1X8 1 1 2, Toronto, ON M5G 1X8, Canada Kagoshima Univ, Fac Med, Dept Neurosurg, Kagoshima 890, Japan Kagoshima Univ Kagoshima Japan 890 Dept Neurosurg, Kagoshima 890, Japan
Titolo Testata:
INTERNATIONAL JOURNAL OF ONCOLOGY
fascicolo: 4, volume: 18, anno: 2001,
pagine: 721 - 727
SICI:
1019-6439(200104)18:4<721:TIFOGA>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VITRO; EXTRACELLULAR-MATRIX; CULTURES; EXPRESSION; INVITRO; NEURONS; PROTEIN; TISSUE; TIMP-1; GELS;
Keywords:
invasion; organotypic slice culture; glial tumor; non-central nervous tumor; gene transfection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Murakami, M Kumamoto Univ, Sch Med, Dept Neurosurg, 1-1-1 Hongo, Kumamoto 860, Japan Kumamoto Univ 1-1-1 Hongo Kumamoto Japan 860 amoto 860, Japan
Citazione:
M. Murakami et al., "The invasive features of glial and non-central nervous system tumor cells are different on organotypic brain slices from newborn rats", INT J ONCOL, 18(4), 2001, pp. 721-727

Abstract

On radiograms, glial tumors are usually seen to invade in a finger-like fashion, while non-central nervous system (CNS) tumors expand in a mass-like fashion. We prepared organotypic brain slice cultures from newborn rats to investigate the invasive behavior of human brain tumors using glial tumor cell lines (U-87MG, U-373MG, U-251MG, and SF-126) and of non-CNS tumors using cell lines; HT-1080 (human malignant fibrosarcoma), RFRF (human lung carcinoma), MIA-PICA (human pancreatic carcinoma), and Colo38 (human malignant:melanoma). We selected an area that was centered at a junction between deep cortical layers and the striatum, punched a hole measuring 0.5-0.7 mm in diameter in this area, implanted different rhodamine-labeled tumor cells atthat site, and observed their invasive behavior under an inverted fluorescent microscope. Over the course of several days, all glioma cells invaded in a finger-like fashion; U-373 MG cells invaded farthest. Non-CNS tumors expanded in mass-like fashion into adjacent areas. Using the slice cultures, we also investigated the regulatory effect on tumor invasion of forced expression of glial fibrillary acidic protein (GFAP) after gene transfection. The forced expression of GFAP rendered U-87MG and HT-1080 cells less invasive. Our results show that organotypic brain slice cultures are an excellent medium for studying the invasive features of glial and non-CNS tumors.

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Documento generato il 18/01/20 alle ore 01:51:02