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Titolo:
In-vitro growth of murine pre-antral follicles after isolation from cryopreserved ovarian tissue
Autore:
Newton, H; Illingworth, P;
Indirizzi:
Westmead Hosp, Dept Reprod Med, Sydney, NSW 2145, Australia Westmead HospSydney NSW Australia 2145 Med, Sydney, NSW 2145, Australia
Titolo Testata:
HUMAN REPRODUCTION
fascicolo: 3, volume: 16, anno: 2001,
pagine: 423 - 429
SICI:
0268-1161(200103)16:3<423:IGOMPF>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG-TERM CULTURE; PREANTRAL FOLLICLES; HUMAN OOCYTES; PRIMORDIAL FOLLICLES; MOUSE OVARIES; INHIBIN-A; MATURATION; SURVIVAL; VITRIFICATION; MICE;
Keywords:
cryopreservation; culture; follicle; ovary;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Newton, H Westmead Hosp, Dept Reprod Med, Sydney, NSW 2145, Australia Westmead Hosp Sydney NSW Australia 2145 ey, NSW 2145, Australia
Citazione:
H. Newton e P. Illingworth, "In-vitro growth of murine pre-antral follicles after isolation from cryopreserved ovarian tissue", HUM REPR, 16(3), 2001, pp. 423-429

Abstract

The low temperature storage of ovarian tissue allows patients at risk of premature menopause to preserve their fecundity. One strategy for harvestingmature oocytes may be to isolate small follicles from the stroma for in-vitro culture. The aim of this study was to assess the survival and growth ofmurine follicles during in-vitro culture after isolation from tissue frozen-thawed in various cryoprotective agents. The effect of different seeding and thawing temperatures was also investigated. Pre-antral follicles 100-135 mum in diameter were isolated from fresh and frozen-thawed tissue and cultured in vitro for 8 days. In the fresh control group 79 +/- 3% of follicles survived in-vitro culture and grew to antral stages. Fewer follicles survived after isolation from tissue cryopreserved in dimethyl sulphoxide (43 +/- 5%) or propylene glycol (24 +/- 2%) and none survived freeze-thawing in glycerol. Lowering the seeding temperature from 5 degrees to -7 degrees or -9 degreesC reduced follicle survival rates from 49 +/- 4% to 26 +/- 1% and28 +/- 3% respectively. If thawing was carried out at 27 degreesC folliclesurvival rate was higher (38 +/- 7%) than at 37 degreesC (26 +/- 2%) or 47degreesC (20 +/- 6%). Follicles surviving 8 days of in-vitro culture were stimulated with human chorionic gonadotrophin. The number of mature oocytesreleased did not differ between any experimental group. The results indicate that follicles isolated from frozen-thawed tissue can be grown to antralsizes and produce mature oocytes. The in-vitro culture system also proved a sensitive method for testing variations in the freeze-thaw protocol.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 22:20:13