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Titolo:
Cloning a calcium channel alpha 2 delta-3 subunit gene from a putative tumor suppressor gene region at chromosome 3p21.1 in conventional renal cell carcinoma
Autore:
Hanke, S; Bugert, P; Chudek, J; Kovacs, G;
Indirizzi:
Univ Heidelberg, Dept Urol, Oncol Mol Lab, D-69120 Heidelberg, Germany Univ Heidelberg Heidelberg Germany D-69120 , D-69120 Heidelberg, Germany
Titolo Testata:
GENE
fascicolo: 1, volume: 264, anno: 2001,
pagine: 69 - 75
SICI:
0378-1119(20010207)264:1<69:CACCA2>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
VONHIPPEL-LINDAU DISEASE; LUNG-CANCER; HOMOZYGOUS DELETION; ALLELE LOSS; FHIT GENE; COMMON; LOCUS; EXPRESSION; FRA3B; ALPHA-1-SUBUNIT;
Keywords:
deletion mapping; BAC contig; positional cloning; mutation analysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Kovacs, G Univ Heidelberg, Dept Urol, Oncol Mol Lab, Neuenheimer Feld 365,D-69120 Heidelberg, Germany Univ Heidelberg Neuenheimer Feld 365 Heidelberg Germany D-69120
Citazione:
S. Hanke et al., "Cloning a calcium channel alpha 2 delta-3 subunit gene from a putative tumor suppressor gene region at chromosome 3p21.1 in conventional renal cell carcinoma", GENE, 264(1), 2001, pp. 69-75

Abstract

We have identified loss of heterozygosity (LOH) of approx. 1 cM region around locus D3S1289 at chromosome 3p21.1 in a conventional renal cell carcinoma (RCC), During construction of a YAC/BAC contig fur this region and shotgun sequencing of BACs 277p5, 55m24 and 428i24, we detected four new microsatellites. We narrowed down the target region by analysing these new loci toless than 100 kb within the BAC 55m24 and subsequently cloned a human calcium channel alpha2 delta -3 subunit gene. This gene is widely expressed in fetal tissues and different types of adult tumors. The exons of the alpha2 delta -3 subunit gene are distributed along approx. 500 kb DNA sequences. As the LOH involved exclusively intronic sequences and sequencing the entirecoding region did not reveal any mutation, the beta2 delta -3 subunit geneis probably not a tumor suppressor gene. (C) 2001 Elsevier Science B.V, All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/08/20 alle ore 00:53:00