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Titolo:
Improved plasmid vectors for the production of multiple fluorescent protein fusions in Bacillus subtilis
Autore:
Feucht, A; Lewis, PJ;
Indirizzi:
Univ Newcastle, Sch Biol & Chem Sci, Callaghan, NSW 2308, Australia Univ Newcastle Callaghan NSW Australia 2308 allaghan, NSW 2308, Australia Univ Oxford, Sir William Dunn Sch Pathol, Oxford OX1 3RE, England Univ Oxford Oxford England OX1 3RE n Sch Pathol, Oxford OX1 3RE, England
Titolo Testata:
GENE
fascicolo: 2, volume: 264, anno: 2001,
pagine: 289 - 297
SICI:
0378-1119(20010221)264:2<289:IPVFTP>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-SPECIFIC TRANSCRIPTION; GENE-EXPRESSION; ASYMMETRIC DIVISION; SPORULATION; LOCALIZATION; FTSZ; SITE; SPOIIE; REPLICATION; MECHANISM;
Keywords:
fluorescent proteins; dual labelling; subcellular localisation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Lewis, PJ Univ Newcastle, Sch Biol & Chem Sci, Callaghan, NSW 2308, Australia Univ Newcastle Callaghan NSW Australia 2308 NSW 2308, Australia
Citazione:
A. Feucht e P.J. Lewis, "Improved plasmid vectors for the production of multiple fluorescent protein fusions in Bacillus subtilis", GENE, 264(2), 2001, pp. 289-297

Abstract

The intrinsically fluorescent green fluorescent protein has been used in many laboratories as a cytalogical marker to monitor protein localisation inlive cells. Multiple spectrally modified mutant versions and novel fluorescent proteins from other species have subsequently been reported and used for labelling cells with multiple fluorescent protein fusions. In this work we report the design and use of vectors containing some of these spectral variants of GFP for use in the Gram positive bacterium Bacillus subtilis. These vectors complement those previously described (Lewis and Marston, 1999. Gene 227, 101-109) to provide a large suite of plasmid vectors for use in this and other related Gram positive organisms. Using these vectors we havebeen able to directly demonstrate the sequential assembly/disassembly of proteins involved in the generation of cellular asymmetry during development. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 13:50:34