Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Mouse phosphoglycerate mutase M and B isozymes: cDNA cloning, enzyme activity assay and mapping
Autore:
Zhang, JX; Yu, L; Fu, Q; Gao, J; Xie, YH; Chen, J; Zhang, PZ; Liu, Q; Zhao, SY;
Indirizzi:
Fudan Univ, Sch Life Sci, Genet Inst, State Key Lab Genet Engn, Shanghai 200433, Peoples R China Fudan Univ Shanghai Peoples R China 200433 nghai 200433, Peoples R China
Titolo Testata:
GENE
fascicolo: 2, volume: 264, anno: 2001,
pagine: 273 - 279
SICI:
0378-1119(20010221)264:2<273:MPMMAB>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
MUSCLE-SPECIFIC SUBUNIT; AMINO-ACID-SEQUENCE; GENE; DEFICIENCY; PGAM;
Keywords:
tissue expression pattern; transient expression; radiation hybrid mapping; Pgam1; Pgam2; Pgam1-ps1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Yu, L Fudan Univ, Sch Life Sci, Genet Inst, State Key Lab Genet Engn, 220 HandanRd, Shanghai 200433, Peoples R China Fudan Univ 220 Handan Rd Shanghai Peoples R China 200433 es R China
Citazione:
J.X. Zhang et al., "Mouse phosphoglycerate mutase M and B isozymes: cDNA cloning, enzyme activity assay and mapping", GENE, 264(2), 2001, pp. 273-279

Abstract

Two mouse cDNAs encoding the non-muscle-specific or brain isoform (type B,Pgam1) and the muscle-specific isoform (type M, Pgam2) of phosphoglyceratemutase (PGAM) were isolated and characterized. Pgam1 contains a 765 bp open reading frame (ORF) coding for a 254-residue protein while Pgam2 containsa 762 bp ORF coding for a 253-residue protein. The deduced proteins of mouse Pgam2 and Pgam2 are highly similar to those of human and rat ( greater than or equal to 93 % similarity). Northern blot analysis showed that the expression patterns of Pgam1 and Pgam2 were distinct. Pgam1 was expressed as a 2.1-kb transcript highly in brain and kidney and moderately in liver, thyroid, stomach and heart, whereas Pgam2 was expressed as a 1.0-kb transcripthighly in muscle, testis and moderately in heart and lung, but was not detectable in the other six tissues examined. Transfecting the cDNA fragments containing the entire ORFs of these two cDNAs into COS7 cells for transientexpression, respectively, the enzyme activities of mouse Pgam1 and Pgam2 were detected to be 2.2-2.5 times of those of COS7 cells and COS7 cells transfected with vector, proving the validity of mouse Pgam1 and Pgam2 cDNAs wereport here. Pgam1 and Pgam2 were assigned to 116.16 cR from D19Mit52 and 29.57 cR from D11Mit129, respectively, by radiation hybrid method. The partial genomic sequence of Pgam2 was determined, which showed that mouse Pgam2consisted at least three exons and two introns. In addition, a pseudogene of Pgam1, Pgam1-ps1, was identified from mouse genomic sequence. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 00:09:23