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Titolo:
Monitoring promoter activity and protein localization in Mycobacterium spp. using green fluorescent protein
Autore:
Cowley, SC; Av-Gay, Y;
Indirizzi:
Univ British Columbia, Dept Med, Div Infect Dis, Vancouver, BC V5Z 3J5, Canada Univ British Columbia Vancouver BC Canada V5Z 3J5 ver, BC V5Z 3J5, Canada
Titolo Testata:
GENE
fascicolo: 2, volume: 264, anno: 2001,
pagine: 225 - 231
SICI:
0378-1119(20010221)264:2<225:MPAAPL>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-EXPRESSION; CELL BIOLOGY; TUBERCULOSIS; CLONING; GFP; BIOSYNTHESIS; MACROPHAGES; PHAGOSOMES; FUSIONS; MARKER;
Keywords:
green fluorescent proteins; transcriptional fusion; translational fusion;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Av-Gay, Y Univ British Columbia, Dept Med, Div Infect Dis, 2733 Heather St, Vancouver, BC V5Z 3J5, Canada Univ British Columbia 2733 Heather St Vancouver BC Canada V5Z 3J5
Citazione:
S.C. Cowley e Y. Av-Gay, "Monitoring promoter activity and protein localization in Mycobacterium spp. using green fluorescent protein", GENE, 264(2), 2001, pp. 225-231

Abstract

Two green fluorescent protein (Gfp) fusion Vectors were constructed for use in Mycobacterium spp. The first plasmid facilitates quantification of mycobacterial promoter activity. The second vector permits construction of translational fusions of mycobacterial proteins to Gfp in order to study subcellular localization including protein secretion. Using this translational fusion construct, we verify that a Gfp fusion to the putative secreted M. tuberculosis protein ChoD is translocated to the extracellular milieu when cloned and expressed in Mycobacterium smegniatis. (C) 2001 Elsevier Science B. V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 22:56:30