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Titolo:
Genomic organization and transcriptional analysis of STDEFICIENS in Solanum tuberosum L
Autore:
Garcia-Maroto, F; Carmona, MJ;
Indirizzi:
Univ Politecn Madrid, ETS Ingenieros Agron, Dept Biotecnol, E-28040 Madrid, Spain Univ Politecn Madrid Madrid Spain E-28040 otecnol, E-28040 Madrid, Spain
Titolo Testata:
GENE
fascicolo: 2, volume: 264, anno: 2001,
pagine: 163 - 171
SICI:
0378-1119(20010221)264:2<163:GOATAO>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
HOMEOTIC GENE APETALA3; FLORAL ORGAN IDENTITY; FLOWER DEVELOPMENT; MADS-BOX; MOLECULAR-CLONING; ARABIDOPSIS; EXPRESSION; DEFICIENS;
Keywords:
DEFICIENS; MADS-box; potato; flower development; transcription start;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Garcia-Maroto, F Univ Almeria, Fac Ciencias Expt, Dept Bioquim, Almeria 04120, Spain Univ Almeria Almeria Spain 04120 , Almeria 04120, Spain
Citazione:
F. Garcia-Maroto e M.J. Carmona, "Genomic organization and transcriptional analysis of STDEFICIENS in Solanum tuberosum L", GENE, 264(2), 2001, pp. 163-171

Abstract

The genomic organization of STDEFICIENS (STDEF), the potato orthologous gene to DEFICIENS (DEF) from Antirrhinum majus and APETALA3 (AP3) from Arabidopsis thaliana, has been investigated. Southern-blot analysis on genomic DNA from dihaploid potato lines, using 5'-gene specific probes, revealed polymorphisms that were consistent with the existence in potato of at least twocopies of STDEF per haploid genome. This was confirmed by the detection ofat least six different STDEF transcripts in the common tetraploid potato S. tuberosum. Genes for two of the STDEF loci, here designated as STDEF-1 and STDEF-2, have been identified as corresponding to the previously described pD13 and pD12 genomic clones, respectively (Garcia-Maroto et al., 1993). In addition we have characterised the transcriptional STDEF unit. The main transcription start has been identified around 90 nt upstream of the putative initiation ATG codon, at a CAAATC motif, conserved in AP3. An additionaltranscription initiation site was detected by 5'-RACE analysis about 300 nt upstream of the main start, which has been confirmed by reverse transcriptase - polymerase chain reaction (RT-PCR) amplification from the longer transcripts. A comparison of the promoter regions for pD12, pD13 and AP3 indicates a similar overall structure, but reveals the existence of a great divergence between pD12 and pD13 in a promoter region that should contain important cis-regulatory elements. This raises the possibility of a differentialregulation for the two STDEF genes. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 21:06:45