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Titolo:
Activated astrocytes in areas of kainate-induced neuronal injury upregulate the expression of the metabotropic glutamate receptors 2/3 and 5
Autore:
Ferraguti, F; Corti, C; Valerio, E; Mion, S; Xuereb, J;
Indirizzi:
Univ Cambridge, Addenbrookes Hosp, Dept Pathol, Cambridge Brain Bank Lab, Cambridge CB2 2QQ, England Univ Cambridge Cambridge England CB2 2QQ Lab, Cambridge CB2 2QQ, England GlaxoWellcome Med Res Ctr, Dept Biol, I-37135 Verona, Italy GlaxoWellcome Med Res Ctr Verona Italy I-37135 ol, I-37135 Verona, Italy Univ Verona, Policlin Borgo Roma, Dept Med & Publ Hlth, Pharmacol Sect, I-37134 Verona, Italy Univ Verona Verona Italy I-37134 , Pharmacol Sect, I-37134 Verona, Italy
Titolo Testata:
EXPERIMENTAL BRAIN RESEARCH
fascicolo: 1, volume: 137, anno: 2001,
pagine: 1 - 11
SICI:
0014-4819(200103)137:1<1:AAIAOK>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
INOSITOL PHOSPHOLIPID HYDROLYSIS; EXCITATORY AMINO-ACIDS; CENTRAL-NERVOUS-SYSTEM; REACTIVE ASTROCYTES; GROWTH-FACTOR; CULTURED ASTROCYTES; RAT HIPPOCAMPUS; UP-REGULATION; CALCIUM OSCILLATIONS; CORTICAL ASTROCYTES;
Keywords:
kainate; gliosis; plasticity; hippocampus; mouse;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Ferraguti, F Univ Oxford, MRC, Anat Neuropharmacol Unit, Mansfield Rd, Oxford OX1 3TH, England Univ Oxford Mansfield Rd Oxford England OX1 3TH 3TH, England
Citazione:
F. Ferraguti et al., "Activated astrocytes in areas of kainate-induced neuronal injury upregulate the expression of the metabotropic glutamate receptors 2/3 and 5", EXP BRAIN R, 137(1), 2001, pp. 1-11

Abstract

All forms of brain injury induce activation of astrocytes, although different types of injury induce different astrocytic responses. Activated astrocytes are characterised by hypertrophy, proliferation and increased expression of glial fibrillary acidic protein (GFAP). However, neither the process by which astrocytes become reactive nor the consequences are well understood. Recently, the application of specific growth factors to primary astrocytic cultures was shown to regulate dramatically the level of expression of the metabotropic glutamate receptors (mGluR) 5 and 3. In the present study, we have used an intracerebroventricular injection of a subconvulsive dose of kainic acid to produce a lesion of CA3a pyramidal neurones in the mouse hippocampus and to investigate whether mGluR expression was altered in reactive astrocytes in vivo. Immunohistochemical analysis showed strong mGluR5 and mGluR2/3 immunoreactivity in glial cells within the area of neuronal loss possessing the morphological feature of activated astrocytes. Double labelling with GFAP confirmed the expression of mGluRs by reactive astrocytes. The mechanical injury produced by the needle insertion in the cerebral cortex also produced enhanced expression of mGluR5 and mGluR2/3 in activated astrocytes proximal to the area of neuronal injury. Our finding of an increased mGluR expression in reactive astrocytes in vivo suggests that transcriptional regulation by specific growth factors on mGluRs is a phenomenon extendible to specific circumstances in vivo and not limited to in vitro models. Identification of the mechanisms of this adaptive plasticity will be central in the understanding of the events leading to neuronal survival and/or death.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 05:33:11