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Titolo:
Evidence for an atrial natriuretic peptide-like gene in plants
Autore:
Vesely, MD; Gower, WR; Perez-Lamboy, G; Overton, RM; Graddy, L; Vesely, DL;
Indirizzi:
James A Haley Vet Hosp, Atrial Natriuret Peptides Res Labs, Tampa, FL 33612 USA James A Haley Vet Hosp Tampa FL USA 33612 s Res Labs, Tampa, FL 33612 USA Univ S Florida, Hlth Sci Ctr, Dept Biochem & Mol Biol, Tampa, FL 33612 USAUniv S Florida Tampa FL USA 33612 Biochem & Mol Biol, Tampa, FL 33612 USA Univ S Florida, Hlth Sci Ctr, Dept Internal Med, Tampa, FL 33612 USA Univ S Florida Tampa FL USA 33612 Dept Internal Med, Tampa, FL 33612 USA Univ S Florida, Hlth Sci Ctr, Dept Physiol, Tampa, FL 33612 USA Univ S Florida Tampa FL USA 33612 Ctr, Dept Physiol, Tampa, FL 33612 USA Univ S Florida, Hlth Sci Ctr, Dept Biophys, Tampa, FL 33612 USA Univ S Florida Tampa FL USA 33612 Ctr, Dept Biophys, Tampa, FL 33612 USA
Titolo Testata:
EXPERIMENTAL BIOLOGY AND MEDICINE
fascicolo: 1, volume: 226, anno: 2001,
pagine: 61 - 65
SICI:
1535-3702(200101)226:1<61:EFAANP>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
CONGESTIVE-HEART-FAILURE; FACTOR PROHORMONE; 3 PEPTIDES; RAT; EXPRESSION; CDNA; IDENTIFICATION; PRECURSOR; SEQUENCES; TERMINUS;
Keywords:
cocaine; apoptosis; TNF-alpha; heart; liver; mouse;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Vesely, DL James A Haley Vet Hosp, Atrial Natriuret Peptides Res Labs, 151,13000 Bruce B Downs Blvd, Tampa, FL 33612 USA James A Haley Vet Hosp 151,13000 Bruce B Downs Blvd Tampa FL USA 33612
Citazione:
M.D. Vesely et al., "Evidence for an atrial natriuretic peptide-like gene in plants", EXP BIOL ME, 226(1), 2001, pp. 61-65

Abstract

The presence of a gene found in the animal kingdom expressing a peptide hormonal system in plants has never been demonstrated. However, there is at least one potential hormonal system in plants (i.e., the atrial natriuretic peptide-like hormonal system) based upon high-performance gel permeation chromatography and radioimmunoaasay evidence, In plants, atrial natriuretic-like peptides enhance the flow of water up stems to leaves and flowers. The present investigation was designed to determine within plants the presence of the atrial natriuretic peptide (ANP) gene as defined by Southern blot hydridization, indicating the presence of the ANP gene sequence, and by Northern blots assessing the ability of this gene to express ANP prohormone mRNA. Southern blots of English ivy (Hedra helix) genomic DNA revealed that theANP gene sequence was present in its roots, stems, and leaves. Northern blot analysis of total plant RNA isolated from leaves, roots, and stems of Hedra helix revealed a single 0.85-kilobase prohormone ANP transcript in stems similar to that detected in rat heart. Semiquantitative analysis suggested that ANP gene expression was less in English ivy compared with that of rat heart atria but similar to the amount found in extra atrial rat tissues when corrected for total RNA when quantitated by 2D scanning. The demonstration of the ANP gene sequences and expression of the ANP-like gene in plantssuggests that plants and animals may have evolved much more similarly thanpreviously thought.

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Documento generato il 25/11/20 alle ore 18:39:22