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Titolo:
Dipeptidyl peptidase IV in two human glioma cell lines
Autore:
Sedo, A; Malik, R; Drbal, K; Lisa, V; Vlasicova, K; Mares, V;
Indirizzi:
Charles Univ, Fac Med 1, Inst Med Chem & Biochem 1, Joint Lab Canc Cell Biol, CR-11636 Prague 1, Czech Republic Charles Univ Prague Czech Republic 1 , CR-11636 Prague 1, Czech Republic Acad Sci Czech Republ, Inst Physiol, Prague, Czech Republic Acad Sci CzechRepubl Prague Czech Republic iol, Prague, Czech Republic Acad Sci Czech Republ, Inst Expt Med, Lab Cell Pathol, Prague, Czech Republic Acad Sci Czech Republ Prague Czech Republic hol, Prague, Czech Republic
Titolo Testata:
EUROPEAN JOURNAL OF HISTOCHEMISTRY
fascicolo: 1, volume: 45, anno: 2001,
pagine: 57 - 63
SICI:
1121-760X(2001)45:1<57:DPIITH>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEASES; HYDROLYSIS; MOLECULE; ASSAY; CD26;
Keywords:
dipeptidyl peptidase IV; glioma cell lines; cell proliferation and differentiation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Sedo, A Charles Univ, Fac Med 1, Inst Med Chem & Biochem 1, Joint Lab CancCell Biol, CR-11636 Prague 1, Czech Republic Charles Univ Prague Czech Republic 1 36 Prague 1, Czech Republic
Citazione:
A. Sedo et al., "Dipeptidyl peptidase IV in two human glioma cell lines", EUR J HIST, 45(1), 2001, pp. 57-63

Abstract

There is growing evidence that dipeptidyl peptidase IV [DPP-IV, EC 3.4.14.5] takes part in the metabolism of biologically active peptides participating in the regulation of growth and transformation of glial cells. However, the knowledge on the DPP-IV expression in human glial and glioma cells is still, very limited. In this study, using histochemical and biochemical techniques, the DPP-IV activity was demonstrated in two commercially available human glioma cell lines of different transformation degree, as represented by U373 astrocytoma (Grade III) and U87 glioblastoma multiforme (Grade IV) lines. Higher total activity of the enzyme, as well as its preferential localisation in the plasma membrane, was observed in U87 cells. Compared to U373 population, U87 cells were morphologically more pleiomorphic, they were cycling at lower rate and expressing less Glial Fibrillary Acidic Protein. The data revealed positive correlation between the degree of transformationof cells and activity of DPP-IV. Great difference in expression of this enzyme, together with the phenotypic differences of cells, makes these lines a suitable standard model for further studies of function of this enzyme inhuman glioma cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 17:32:18