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Titolo:
Identifying unknown game species: experience with nucleotide sequencing ofthe mitochondrial cytochrome b gene and a subsequent basic local alignmentsearch tool search
Autore:
Brodmann, PD; Nicholas, G; Schaltenbrand, P; Ilg, EC;
Indirizzi:
Kantonales Lab Basel Stadt, CH-4056 Basel, Switzerland Kantonales Lab Basel Stadt Basel Switzerland CH-4056 Basel, Switzerland
Titolo Testata:
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
fascicolo: 4, volume: 212, anno: 2001,
pagine: 491 - 496
SICI:
1438-2377(2001)212:4<491:IUGSEW>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE-CHAIN-REACTION; HEAT-TREATED MEAT; PCR-RFLP ANALYSIS; RELIABLE METHOD; DNA-PATTERNS; IDENTIFICATION; PRODUCTS; PROBES; FISH; FOOD;
Keywords:
species identification; cytochrome b; polymerase chain reaction; sequence identity; cervidae;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Brodmann, PD Kantonales Lab Basel Stadt, Kannenfeldstr 2, CH-4056 Basel, Switzerland Kantonales Lab Basel Stadt Kannenfeldstr 2 Basel Switzerland CH-4056
Citazione:
P.D. Brodmann et al., "Identifying unknown game species: experience with nucleotide sequencing ofthe mitochondrial cytochrome b gene and a subsequent basic local alignmentsearch tool search", EUR FOOD RE, 212(4), 2001, pp. 491-496

Abstract

Game meat is often a target: for fraudulent labelling. Polymerase chain reaction (PCR) analysis on the mitochondrial cytochrome b sequence with subsequent restriction fragment length polymorphism is the most widely used method of identifying meat species. The lack of reference meat species and the possibility of point mutations affecting the typical restriction pattern ofa species sometimes results in an analytical uncertainty. Nowadays, sequencing of the PCR fragment with a subsequent search in an internet-accessibledatabase can avoid these problems. The database search results in a list of sequences in order of the highest percentage of correspondence. In this work it is shown what correspondences within different vertebrate classes, orders, families and species can be expected. Classing with the correct genus is possible, at least for game meal.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 02:00:31