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Titolo:
Routine cryopreservation of kiwifruit (Actinidia spp) germplasm by encapsulation-dehydration: Importance of plant growth regulators
Autore:
Bachiri, Y; Song, GQ; Plessis, P; Shoar-Ghaffari, A; Rekab, T; Morisset, C;
Indirizzi:
Univ Paris 06, Lab Cryobiol Vegetale, F-75252 Paris, France Univ Paris 06Paris France F-75252 obiol Vegetale, F-75252 Paris, France China Agr Univ, Plant Physiol & Biochem Lab, Beijing 100094, Peoples R China China Agr Univ Beijing Peoples R China 100094 ng 100094, Peoples R China
Titolo Testata:
CRYO-LETTERS
fascicolo: 1, volume: 22, anno: 2001,
pagine: 61 - 74
SICI:
0143-2044(200101/02)22:1<61:RCOK(S>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VITRO PLANTLETS; APPLE SHOOT TIPS; THERMAL-ANALYSIS; SOMATIC EMBRYOS; APICES; REGENERATION; DESICCATION; PEAR; BUDS;
Keywords:
Actinidia; Cryopreservation; dehydration; differential scanning calorimetry (DSC); encapsulation; sucrose;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Morisset, C Univ Paris 06, Lab Cryobiol Vegetale, 12 Rue Cuvier, F-75252 Paris, France Univ Paris 06 12 Rue Cuvier Paris France F-75252 aris, France
Citazione:
Y. Bachiri et al., "Routine cryopreservation of kiwifruit (Actinidia spp) germplasm by encapsulation-dehydration: Importance of plant growth regulators", CRYO-LETT, 22(1), 2001, pp. 61-74

Abstract

The encapsulation-dehydration protocol was optimized for an in vitro cultured hybrid Actinidia arguta x A, deliciosa. Shoot tips from 14-d reactivated mononodal microcuttings were embedded, transferred to liquid culture medium whose sucrose concentration was daily increased (0.3, 0.5, 0.75 M) and then kept at 0.75 M for 2 or 4 d. Dehydration on silica gel was monitored to20+/-1.5% residual water content (dry weight basis), allowing direct quenching in liquid nitrogen and rewarming at room temperature. Differential scanning calorimetry analysis underlined the importance of reversible glass transition in shoots for survival. Regrowth ranged from 85% to 95%. Growing shoot tips showed no phenotypic abnormalities. Rooting was also achieved. This method was routinely applied to diploid A. chinensis and A. eriantha, and to several diploid hybrids, yielding over 70% regrowth. A slight decreasein sucrose molarity (0.65 M) allowed tetraploid A. chinensis and A. chrysantha x A. arguta to survive dehydration, but not quenching in LN. I;or A. deliciosa cv Hayward and cv Tomuri, normal regrowth after cryopreservation was achieved only after modification of the pre- and post-culture media, highlighting the importance of monitoring plant growth regulator balance, principally at the post-thaw recovery step.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/05/20 alle ore 14:48:40