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Titolo:
Monosaccharide uptake in common carp (Cyprinus carpio) EPC cells is mediated by a facilitative glucose carrier
Autore:
Teerijoki, H; Krasnov, A; Pitkanen, TI; Molsa, H;
Indirizzi:
Univ Kuopio, Inst Appl Biotechnol, FIN-70211 Kuopio, Finland Univ Kuopio Kuopio Finland FIN-70211 otechnol, FIN-70211 Kuopio, Finland
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
fascicolo: 3, volume: 128, anno: 2001,
pagine: 483 - 491
SICI:
1096-4959(200103)128:3<483:MUICC(>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
EEL ANGUILLA-ROSTRATA; TRANSPORTER GENE; SUGAR TRANSPORTERS; HEXOSE-TRANSPORT; SEQUENCE; ERYTHROCYTES; EXPRESSION; METABOLISM; SYSTEM; FAMILY;
Keywords:
glucose metabolism; glucose transporter; common carp; EPC cells; kinetics; gene cloning;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Krasnov, A Univ Kuopio, Inst Appl Biotechnol, POB 1627, FIN-70211 Kuopio, Finland Univ Kuopio POB 1627 Kuopio Finland FIN-70211 Kuopio, Finland
Citazione:
H. Teerijoki et al., "Monosaccharide uptake in common carp (Cyprinus carpio) EPC cells is mediated by a facilitative glucose carrier", COMP BIOC B, 128(3), 2001, pp. 483-491

Abstract

In most animal cells, transport of monosaccharides across the plasma membrane is mediated by glucose transporters (GLUT). Mammals express at least five distinct transporters (GLUTs 1-5), which are well characterised both functionally and genetically In contrast, the glucose transport system of fishremains poorly studied. Here we report studies of hexose uptake in carp EPC cells and cloning of a glucose transporter cDNA from these cells. Transport of radio-labelled methylglucose (3-OMG) followed Michaelis-Menten kinetics with a K-m value (8.5 mM) similar to that of mammalian cells. The inhibition of transport by cytochalasin B and phloretin, but not by phloridzin orcyanide, strongly suggested the existence of a facilitative carrier. D-Glucose, 2-deoxyglucose, 3-OMG, D-mannose and D-xylose were competitive inhibitors of 3-OMG uptake, while L-glucose, mannitol, D-fructose, D-ribose and sucrose did not compete with 3-OMG. We cloned a carp glucose transporter (CyiGLUT1), using RT-PCR and RACE strategies. CyiGLUT1 was different from known carp and zebrafish EST sequences. The complete cDNA (3060 bp) contained one open reading frame encoding a predicted protein of 478 amino acids. The deduced amino acid sequence shared 78% identity with mammalian and avian GLUT1 proteins. Key amino acids involved in substrate selection and catalysisof mammalian GLUTs were conserved in the carp transporter. (C) 2001 Elsevier Science inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 18:00:25