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Titolo:
Cloning of glycerol-3-phosphate dehydrogenase cDNAs from two fish species and effect of temperature on enzyme expression in rainbow smelt (Osmerus mordax)
Autore:
Ewart, KV; Richards, RC; Driedzic, WR;
Indirizzi:
Natl Res Council Canada, Atlantic Reg Lab, Inst Marine Biosci, Halifax, NSB3H 3Z1, Canada Natl Res Council Canada Halifax NS Canada B3H 3Z1 ifax, NSB3H 3Z1, Canada Mem Univ Newfoundland, Ctr Ocean Sci, St Johns, NF A1C 5S7, Canada Mem Univ Newfoundland St Johns NF Canada A1C 5S7 ohns, NF A1C 5S7, Canada
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
fascicolo: 3, volume: 128, anno: 2001,
pagine: 401 - 412
SICI:
1096-4959(200103)128:3<401:COGDCF>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLYCEROL PHOSPHATE DEHYDROGENASE; SN-GLYCEROL-3-PHOSPHATE DEHYDROGENASE; SACCHAROMYCES-CEREVISIAE; ANTIFREEZE PROTEINS; STRESS; RESPONSES; SEQUENCE; TELEOST; GENE;
Keywords:
rainbow smelt; Osmerus mordax; glycerol; glycerol-3-phosphate dehydrogenase; temperature; gene expression; freeze resistance; Atlantic salmon; Salmo salar;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Ewart, KV Natl Res Council Canada, Atlantic Reg Lab, Inst Marine Biosci, 1411 OxfordSt, Halifax, NS B3H 3Z1, Canada Natl Res Council Canada 1411 Oxford St Halifax NS Canada B3H 3Z1
Citazione:
K.V. Ewart et al., "Cloning of glycerol-3-phosphate dehydrogenase cDNAs from two fish species and effect of temperature on enzyme expression in rainbow smelt (Osmerus mordax)", COMP BIOC B, 128(3), 2001, pp. 401-412

Abstract

Rainbow smelt (Osmerus mordax) can accumulate extreme levels of glycerol in their blood during winter. Low temperatures are required for glycerol accumulation in smelt blood and the enzyme glycerol-3-phosphate dehydrogenase (GPDH) has been suggested to play a role in glycerol production/concentration in this species. In the present study, cDNA sequences encoding glycerol-3-phosphate dehydrogenase (GPDH) from rainbow smelt and Atlantic salmon (Salmo salar) were cloned. The encoded GPDH protein sequences were very similar to one another (88% identity). Using RT-PCR, GPDH mRNA was detected in skin, gill, heart, head kidney, brain and liver from both salmon and smelt obtained in December. However, GPDH was not detected in salmon intestine and spleen or in smelt intestine. Examination of GPDH expression in smelt liverduring February by Northern blotting revealed temperature regulation. Elevation of the temperature resulted in a significant decrease in liver GPDH transcript level. Serum glycerol levels decreased concomitantly. These findings suggest a role for GPDH in the accumulation of glycerol in smelt at lowtemperatures. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 13:20:50