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Titolo:
Altered cardiac sarcoplasmic reticulum function of intact myocytes of rat ventricle during metabolic inhibition
Autore:
Overend, CL; Eisner, DA; ONeill, SC;
Indirizzi:
Univ Manchester, Dept Med, Manchester M13 9PT, Lancs, England Univ Manchester Manchester Lancs England M13 9PT M13 9PT, Lancs, England
Titolo Testata:
CIRCULATION RESEARCH
fascicolo: 2, volume: 88, anno: 2001,
pagine: 181 - 187
SICI:
0009-7330(20010202)88:2<181:ACSRFO>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
ISOLATED FERRET HEARTS; INTRACELLULAR CALCIUM; CONTRACTILE FAILURE; CA2+ RELEASE; RYANODINE RECEPTOR; CELLS; MUSCLE; PH; TETRACAINE; MECHANISM;
Keywords:
cardiac; sarcoplasmic reticulum; calcium; metabolic inhibition;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: O'Neill, SC Univ Manchester, Dept Med, 1-525 Stopford Bldg,Oxford Rd, Manchester M13 9PT, Lancs, England Univ Manchester 1-525 Stopford Bldg,Oxford Rd Manchester Lancs England M13 9PT
Citazione:
C.L. Overend et al., "Altered cardiac sarcoplasmic reticulum function of intact myocytes of rat ventricle during metabolic inhibition", CIRCUL RES, 88(2), 2001, pp. 181-187

Abstract

Changes in the behavior of the sarcoplasmic reticulum (SR) in rat ventricular myocytes were investigated under conditions of metabolic inhibition using laser-scanning confocal microscopy to measure intracellular Ca2+ and theperforated patch-clamp technique to measure SR Ca2+ content. Metabolic inhibition had several effects on SR function, including reduced frequency of spontaneous releases of Ca2+ (sparks and waves of Ca2+-induced Ca2+ release), increased SR Ca2+ content (79.4+/-5.7 to 115.2+/-6.6 mu mol/L cell volume [mean+/-SEM; P<0.001]), and, after a wave of Ca2+ release, slower reuptake of Ca2+ into the SR (rate constant of fall of Ca2+ reduced from 8.5+/-1.1s(-1) in control to 5.2+/-0.4 s(-1) in metabolic inhibition [P<0.01]). Inhibition of L-type Ca2+ channels with Cd2+ (100 mu mol/L) did not reproduce the effects of metabolic inhibition on spontaneous Ca2+ sparks. These results are evidence of inhibition of both Ca2+ release and reuptake mechanisms. Reduced frequency of release could be attributable to either of these effects, but the increased SR Ca2+ content at the time of reduced frequency of spontaneous release of Ca2+ shows that the dominant effect of metabolic inhibition is to inhibit release of Ca2+ from the SR, allowing the accumulation of greater than normal amounts of Ca2+ In the context of ischemia, this extra accumulation of Ca2+ would present a risk of potentially arrhythmogenic, spontaneous release of Ca2+ on reperfusion of the tissue.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 23:31:19